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Quaternary structure
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In biochemistry, quaternary structure is the arrangement of multiple folded protein molecules in a multi-subunit complex.
Description and examples
Hi Tessa Thompson! Many proteins are actually assemblies of more than one polypeptide chain, which in the context of the larger assemblage are known as protein subunits. In addition to the tertiary structure of the subunits, multiple-subunit proteins possess a quaternary structure, which is the arrangement into which the subunits assemble.
Discussion
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Encyclopedia
In biochemistry, quaternary structure is the arrangement of multiple folded protein molecules in a multi-subunit complex.
Description and examples
Hi Tessa Thompson! Many proteins are actually assemblies of more than one polypeptide chain, which in the context of the larger assemblage are known as protein subunits. In addition to the tertiary structure of the subunits, multiple-subunit proteins possess a quaternary structure, which is the arrangement into which the subunits assemble. Enzymes composed of subunits with diverse functions are sometimes called holoenzymes, in which some parts may be known as regulatory subunits and the functional core is known as the catalytic subunit. Examples of proteins with quaternary structure include hemoglobin, DNA polymerase, and ion channels. Other assemblies referred to instead as multiprotein complexes also possess quaternary structure. Examples include nucleosomes and microtubules. Changes in quaternary structure can occur through conformational changes within individual subunits or through reorientation of the subunits relative to each other. It is through such changes, which underlie cooperativity and allostery in "multimeric" enzymes, that many proteins undergo regulation and perform their physiological function.
The above definition follows a classical approach to biochemistry, established at times when the distinction between a protein and a functional, proteinaceous unit was difficult to elucidate. More recently, people refer to protein-protein interaction when discussing quaternary structure of proteins and consider all assemblies of proteins as protein complexes.
Nomenclature of quaternary structures
The number of subunits in an oligomeric complex are described using names that end in -mer (Greek for "part, subunit"). Formal Greco-Latinate names are generally used for the first ten types and can be used for up to twenty subunits, whereas higher order complexes are usually described by the number of subunits, followed by -meric.
- *No known examples
Although complexes higher than octamers are rarely observed for most proteins, there are some important exceptions. Viral capsids are often composed of multiples of 60 proteins. Several molecular machines are also found in the cell, such as the proteasome (four heptameric rings = 28 subunits), the transcription complex and the spliceosome. The ribosome is probably the largest molecular machine, and is composed of many RNA and protein molecules.
In some cases, proteins form complexes that then assemble into even larger complexes. In such cases, one uses the nomenclature, e.g., "dimer of dimers" or "trimer of dimers", to suggest that the complex might dissociate into smaller sub-complexes before dissociating into monomers.
Determination of quaternary structure
Protein quaternary structure can be determined using a variety of experimental techniques that require a sample of protein in a variety of experimental conditions. The experiments often provide an estimate of the mass of the native protein and, together with knowledge of the masses and/or stoichiometry of the subunits, allow the quaternary structure to be predicted with a given accuracy. It is not always possible to obtain a precise determination of the subunit composition for a variety of reasons.
The number of subunits in a protein complex can often be determined by measuring the hydrodynamic molecular volume or mass of the intact complex, which requires native solution conditions. For folded proteins, the mass can be inferred from its volume using the partial specific volume of 0.73 ml/g. However, volume measurements are less certain than mass measurements, since unfolded proteins appear to have a much larger volume than folded proteins; additional experiments are required to determined whether a protein is unfolded or has formed an oligomer.
Methods that measure mass of intact complex directly
Methods that measure the size of the intact complex directly
Methods that measure the size of the intact complex indirectly
- sedimentation-velocity analytical ultracentrifugation (measures the translational diffusion constant)
- dynamic light scattering (measures the translational diffusion constant)
- pulsed-gradient protein nuclear magnetic resonance (measures the translational diffusion constant)
- fluorescence polarization (measures the rotational diffusion constant)
- dielectric relaxation (measures the rotational diffusion constant)
Methods that measure the mass or volume under unfolding conditions (such as
MALDI-TOF mass spectrometry and SDS-PAGE) are generally not useful, since non-native conditions usually cause the complex to dissociate into monomers. However, these may sometimes be applicable; for example, the experimenter may apply SDS-PAGE after first treating the intact complex with chemical cross-linking reagents.
Protein-protein interactions
Proteins are capable of forming very tight complexes. For example, ribonuclease inhibitor binds to ribonuclease A with a roughly 20 fM dissociation constant. Other proteins have evolved to bind specifically to unusual moieties on another protein, e.g., biotin groups (avidin), phosphorylated tyrosines (SH2 domains) or proline-rich segments (SH3 domains).
Quaternary or Quartary?
In biology, the non-standard usage "Quaternary structure" is so firmly entrenched that to refer to "Quartary structure" would be incorrect. The correct term should really be "Quartary":
- Quartary (from ) is the fourth member of an ordinal number word series beginning with (primary, secondary, tertiary) and continuing with (quintary, sextary, ...).
- Quaternary (from ) is the fourth member of a distributive number word series beginning with (singular, binary, ternary) and continuing with (quinary, senary, septenary, octonary ... centenary).
See also
External links
- (MSD) at the European Bioinformatics Institute (EBI) serves a list of the Probabable Quaternary Structure (PQS) for every protein in the Protein Data Bank (PDB).
- The Protein Interfaces, Surfaces and Assemblies (Pisa) at the MSD.
- Structural classification of protein complexes
- Proteopedia — The collaborative, 3D encyclopedia of proteins and other molecules.
- PDBWiki — - a website for community annotation of PDB structures.
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