BAZ1B
Encyclopedia
Tyrosine-protein kinase BAZ1B is an enzyme
that in humans is encoded by the BAZ1B gene
.
s have been used in the study of BAZ1B function. A conditional
knockout mouse
line, called Baz1btm2a(KOMP)Wtsi, was generated as part of the International Knockout Mouse Consortium
program — a high-throughput mutagenesis
project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.
Six significant phenotypes were reported:
, TOP2B
, SMARCC2
, SMARCC1
, SMARCB1
, SUPT16H
and Calcitriol receptor
.
Enzyme
Enzymes are proteins that catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates...
that in humans is encoded by the BAZ1B gene
Gene
A gene is a molecular unit of heredity of a living organism. It is a name given to some stretches of DNA and RNA that code for a type of protein or for an RNA chain that has a function in the organism. Living beings depend on genes, as they specify all proteins and functional RNA chains...
.
Animal models
Model organismModel organism
A model organism is a non-human species that is extensively studied to understand particular biological phenomena, with the expectation that discoveries made in the organism model will provide insight into the workings of other organisms. Model organisms are in vivo models and are widely used to...
s have been used in the study of BAZ1B function. A conditional
Conditional gene knockout
Conditional gene knockout is a relatively new technique, an offshoot of gene knockout technology with the exception that a specific target gene is eliminated from a single organ in the body rather than the whole body as gene knockout technology would entail...
knockout mouse
Knockout mouse
A knockout mouse is a genetically engineered mouse in which researchers have inactivated, or "knocked out," an existing gene by replacing it or disrupting it with an artificial piece of DNA...
line, called Baz1btm2a(KOMP)Wtsi, was generated as part of the International Knockout Mouse Consortium
International Knockout Mouse Consortium
The International Knockout Mouse Consortium is a scientific endeavour to produce a collection of mouse embryonic stem cell lines that together lack every gene in the genome, and then to distribute the cells to scientific researchers to create knockout mice to study...
program — a high-throughput mutagenesis
Mutagenesis
Mutagenesis is a process by which the genetic information of an organism is changed in a stable manner, resulting in a mutation. It may occur spontaneously in nature, or as a result of exposure to mutagens. It can also be achieved experimentally using laboratory procedures...
project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.
| Characteristic | Abnormal |
|---|---|
| Homozygote viability | Yes |
| Body weight Body weight The term body weight is used in daily English speech as well as in the contexts of biological and medical sciences to describe the mass of an organism's body. Body weight is measured in kilograms throughout the world, although in some countries it is still measured in pounds or stones and pounds... |
Yes |
| Hair follicle Hair follicle A hair follicle is a skin organ that produces hair. Hair production occurs in phases, including a growth phase , and cessation phase , and a rest phase . Stem cells are principally responsible for the production of hair.... cycling |
No |
| Anxiety Anxiety Anxiety is a psychological and physiological state characterized by somatic, emotional, cognitive, and behavioral components. The root meaning of the word anxiety is 'to vex or trouble'; in either presence or absence of psychological stress, anxiety can create feelings of fear, worry, uneasiness,... |
No |
| Modified SHIRPA SHIRPA SHIRPA is a standardized set of experimental procedures used by scientists to characterize the phenotype of genetically modified laboratory mice... |
No |
| Grip strength Grip strength Grip strength is the force applied by the hand to pull on or suspend from objects and is a specific part of hand strength. Optimum-sized objects permit the hand to wrap around a cylindrical shape with a diameter from one to three inches. Stair rails are an example of where shape and diameter are... |
No |
| Dysmorphology | No |
| Indirect calorimetry | Yes |
| Glucose tolerance test Glucose tolerance test A glucose tolerance test is a medical test in which glucose is given and blood samples taken afterward to determine how quickly it is cleared from the blood. The test is usually used to test for diabetes, insulin resistance, and sometimes reactive hypoglycemia and acromegaly, or rarer disorders of... |
No |
| Auditory brainstem response Auditory Brainstem Response The auditory brainstem response is an auditory evoked potential extracted from ongoing electrical activity in the brain and recorded via electrodes placed on the scalp. The resulting recording is a series of vertex positive waves of which I through V are evaluated... |
No |
| DEXA | Yes |
| Radiography Radiography Radiography is the use of X-rays to view a non-uniformly composed material such as the human body. By using the physical properties of the ray an image can be developed which displays areas of different density and composition.... |
Yes |
| Body temperature | No |
| Eye morphology Morphology (biology) In biology, morphology is a branch of bioscience dealing with the study of the form and structure of organisms and their specific structural features.... |
No |
| Heart weight | No |
| Heart histology | No |
| Histology Histology Histology is the study of the microscopic anatomy of cells and tissues of plants and animals. It is performed by examining cells and tissues commonly by sectioning and staining; followed by examination under a light microscope or electron microscope... |
No |
| Clinical chemistry | No |
| Plasma Blood plasma Blood plasma is the straw-colored liquid component of blood in which the blood cells in whole blood are normally suspended. It makes up about 55% of the total blood volume. It is the intravascular fluid part of extracellular fluid... immunoglobulins |
No |
| Haematology | No |
| Peripheral blood lymphocytes Peripheral Blood Lymphocytes Peripheral blood lymphocytes are mature lymphocytes that circulate in the blood, rather than localising to organs . They comprise T cells, NK cells and B cells.... |
No |
| Micronucleus test Micronucleus test A micronucleus test is a test used in toxicological screening for potential genotoxic compounds. There are two major versions of this test, one in vivo and the other In vitro. The in vivo test normally uses mouse bone marrow or mouse peripheral blood. Micronuclei were first used to quantify... |
No |
| Salmonella Salmonella Salmonella is a genus of rod-shaped, Gram-negative, non-spore-forming, predominantly motile enterobacteria with diameters around 0.7 to 1.5 µm, lengths from 2 to 5 µm, and flagella which grade in all directions . They are chemoorganotrophs, obtaining their energy from oxidation and reduction... infection |
Yes |
| Citrobacter Citrobacter Citrobacter is a genus of Gram-negative coliform bacteria in the Enterobacteriaceae family.The species C. amalonaticus, C. koseri, and C. freundii use solely citrate as a carbon source... infection |
No |
| All tests and analysis from | |
Six significant phenotypes were reported:
- Less homozygous mutant mice survived to weaningWeaningWeaning is the process of gradually introducing a mammal infant, either human or animal, to what will be its adult diet and withdrawing the supply of its mother's milk.The process takes place only in mammals, as only mammals produce milk...
than expected. - MutantMutantIn biology and especially genetics, a mutant is an individual, organism, or new genetic character, arising or resulting from an instance of mutation, which is a base-pair sequence change within the DNA of a gene or chromosome of an organism resulting in the creation of a new character or trait not...
mice had decreased body weightBody weightThe term body weight is used in daily English speech as well as in the contexts of biological and medical sciences to describe the mass of an organism's body. Body weight is measured in kilograms throughout the world, although in some countries it is still measured in pounds or stones and pounds...
s compared to wildtype control mice. - Mutant mice showed increased activity, VO2 and energy expenditure, determined by indirect calorimetry.
- Radiography found teeth abnormalities.
- Dual-energy X-ray absorptiometry (DEXA) showed mutant female mice had a decrease in bone mineralBone mineralBone mineral is the inorganic component of bone. Bone mineral is formed from carbonated hydroxyapatite with lower crystallinity....
density and content. - Male heterozygous mice had higher bacterial counts after SalmonellaSalmonellaSalmonella is a genus of rod-shaped, Gram-negative, non-spore-forming, predominantly motile enterobacteria with diameters around 0.7 to 1.5 µm, lengths from 2 to 5 µm, and flagella which grade in all directions . They are chemoorganotrophs, obtaining their energy from oxidation and reduction...
infection.
Interactions
BAZ1B has been shown to interact with CHAF1ACHAF1A
Chromatin assembly factor 1 subunit A is a protein that in humans is encoded by the CHAF1A gene.-Interactions:CHAF1A has been shown to interact with ASF1B, ASF1A, MBD1, Bloom syndrome protein and CBX5.-Further reading:...
, TOP2B
TOP2B
DNA topoisomerase 2-beta is an enzyme that in humans is encoded by the TOP2B gene.-Interactions:TOP2B has been shown to interact with BAZ1B, HDAC1, Histone deacetylase 2, T-cell surface glycoprotein CD3 epsilon chain, Small ubiquitin-related modifier 1 and P53.-Further reading:...
, SMARCC2
SMARCC2
SWI/SNF complex subunit SMARCC2 is a protein that in humans is encoded by the SMARCC2 gene.-Interactions:SMARCC2 has been shown to interact with BAZ1B and SMARCA4.-External links:...
, SMARCC1
SMARCC1
SWI/SNF complex subunit SMARCC1 is a protein that in humans is encoded by the SMARCC1 gene.-Interactions:SMARCC1 has been shown to interact with BAZ1B, SIN3A, SMARCA2, ING1, SMARCB1 and SMARCA4.-External links:...
, SMARCB1
SMARCB1
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 is a protein that in humans is encoded by the SMARCB1 gene.-Interactions:...
, SUPT16H
SUPT16H
FACT complex subunit SPT16 is a protein that in humans is encoded by the SUPT16H gene.-Further reading:...
and Calcitriol receptor
Calcitriol receptor
The calcitriol receptor, also known as the vitamin D receptor and also known as NR1I1 , is a member of the nuclear receptor family of transcription factors...
.