Trypticase soy agar
Encyclopedia
Trypticase soy agar is a bacteria
l growth medium.
The medium contains enzymatic digests of casein
and soybean
meal which provides amino acid
s and other nitrogenous substances making it a nutritious medium for a variety of organisms. Glucose
is the energy source. Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH. Agar extracted from any number of organisms is used as a gelling agent.
The medium may be supplemented with blood to facilitate the growth of more fastidious
bacteria or antimicrobial agents to permit the selection of various microbial groups from pure flora. As with any media, minor changes may be made to suit specific circumstances.
One liter of the agar contains:
Bacteria
Bacteria are a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria have a wide range of shapes, ranging from spheres to rods and spirals...
l growth medium.
The medium contains enzymatic digests of casein
Casein
Casein is the name for a family of related phosphoprotein proteins . These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 60% and 65% of the proteins in human milk....
and soybean
Soybean
The soybean or soya bean is a species of legume native to East Asia, widely grown for its edible bean which has numerous uses...
meal which provides amino acid
Amino acid
Amino acids are molecules containing an amine group, a carboxylic acid group and a side-chain that varies between different amino acids. The key elements of an amino acid are carbon, hydrogen, oxygen, and nitrogen...
s and other nitrogenous substances making it a nutritious medium for a variety of organisms. Glucose
Glucose
Glucose is a simple sugar and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate...
is the energy source. Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH. Agar extracted from any number of organisms is used as a gelling agent.
The medium may be supplemented with blood to facilitate the growth of more fastidious
Growth medium
A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens.There are different types of media for growing different types of cells....
bacteria or antimicrobial agents to permit the selection of various microbial groups from pure flora. As with any media, minor changes may be made to suit specific circumstances.
One liter of the agar contains:
- 15 g TryptoneTryptoneTryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.Tryptone is commonly used in microbiology to produce Lysogeny broth for the growth of E. coli and other microorganisms...
- 5 g Soytone - enzymatic digest of soybean meal
- 5 g Sodium ChlorideSodium chlorideSodium chloride, also known as salt, common salt, table salt or halite, is an inorganic compound with the formula NaCl. Sodium chloride is the salt most responsible for the salinity of the ocean and of the extracellular fluid of many multicellular organisms...
- 15 g AgarAgarAgar or agar-agar is a gelatinous substance derived from a polysaccharide that accumulates in the cell walls of agarophyte red algae. Throughout history into modern times, agar has been chiefly used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture medium...