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Artificial selection
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Artificial selection is the intentional breeding for certain traits, or combinations of traits, over others, and is synonymous with "Selective breeding". It was originally defined by Charles Darwin in contrast to the process of natural selection, in which the differential reproduction of organisms with certain traits is attributed to improved survival and/or reproductive ability ("Darwinian fitness") in the natural habitat of the organism. Artificial selection that produces an undesirable outcome from a human perspective is sometimes called negative selection (but note that this term has a better-established meaning as a type of natural selection; see negative selection). Artificial selection can also be unintentional; it is thought that domestication of crops by early humans was largely unintentional.
Historical developmentCharles Darwin coined the term as an illustration of his proposed wider process of natural selection. Darwin noted that many domesticated animals and plants had special properties that were developed by intentionally encouraging the breeding potential of individuals who both possessed desirable characteristics, and discouraging the breeding of individuals who had less desirable characteristics.
Laboratory usageThe deliberate exploitation of selective power has become common in experimental biology, particularly in microbiology and genetics. In a ubiquitous laboratory technique in genetic engineering, genes are introduced into cells in cell culture, usually bacteria, on a small circular DNA molecule called a plasmid in a process called transfection. The gene of interest is accompanied on the plasmid by a reporter gene, or "selectable marker", which encodes a specific trait such as antibiotic resistance or ability to grow in high salt concentrations. The cells can then be cultured in an environment that would kill normal cells, but is hospitable to those that have taken up and expressed the genes on the plasmid. In this way expression of the reporter gene serves as a signal that the gene of interest is also being expressed in the cells.
Another technique used in drug development uses an iterative selective process called in vitro selection to evolve aptamers, or nucleic acid fragments capable of binding specific organic compounds with high binding affinity.
Studies in evolutionary physiology, behavioral genetics, and other areas of organismal biology have also made use of deliberate artificial selection, though longer generation times and greater difficulty in breeding can make such projects challenging in vertebrates.
Online experiments in artificial selection
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