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Apyrase

 

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Apyrase



 
 
Apyrase is a calcium
Calcium

Calcium is the chemical element with the symbol Ca and atomic number 20. It has an atomic mass of 40.078 amu. Calcium is a soft grey alkaline earth metal, and is the fifth most abundant element by mass in the earth's Crust ....
-activated plasma membrane-bound enzyme
Enzyme

Enzymes are biomolecules that catalysis chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules at the beginning of the process are called Substrate , and the enzyme converts them into different molecules, the products....
 (magnesium can also activate it) that catalyses the hydrolysis
Hydrolysis

Hydrolysis is a chemical reaction during which one or more water are split into hydrogen and hydroxide ions which may go on to participate in further reactions....
 of ATP
Adenosine triphosphate

This article is about the chemical used by cells as an energy carrier. For other uses, see ATP .Adenosine-5'-triphosphate is a multifunctional nucleotide, and plays an important role in cell biology as a coenzyme that is the "molecule unit of currency" of intracellular energy transfer....
 to yield AMP
Adenosine monophosphate

Adenosine monophosphate , also known as 5'-adenylic acid, is a nucleotide that is found in RNA. It is an ester of phosphoric acid and the nucleoside adenosine....
 and inorganic phosphate. Two isoenzymes are found in commercial preparations from S. tuberosum. One with a higher ratio of substrate selectivity for ATP:ADP (approx 10) and another with no selectivity (ratio 1).

It can also act on ADP
Adenosine diphosphate

Adenosine diphosphate, abbreviated ADP, is a nucleotide. It is an ester of pyrophosphoric acid with the nucleoside adenosine. ADP consists of the pyrophosphate Functional group, the pentose sugar ribose, and the nucleobase adenine....
 and other nucleoside
Nucleoside

Nucleosides are glycosylamines consisting of a nucleobase bound to a ribose or deoxyribose sugar. Examples of these include cytidine, uridine, adenosine, guanosine, thymidine and inosine....
 triphosphates and diphosphates with the general reaction being NTP -> NDP + Pi -> NMP + 2Pi.

It is the enzyme
Enzyme

Enzymes are biomolecules that catalysis chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules at the beginning of the process are called Substrate , and the enzyme converts them into different molecules, the products....
 released by female mosquito
Mosquito

Mosquitoes are common flying insects in the family Culicidae that are found around the world. There are about 3,500 species. They have a pair of scaled wings, a pair of halteres, a slender body, and six long legs....
es to prevent blood clots when they suck blood.

The activity of apyrase at pH 7.5 in a Tris Buffer is approximately 80% of the activity at pH 6.5.








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Encyclopedia


Apyrase is a calcium
Calcium

Calcium is the chemical element with the symbol Ca and atomic number 20. It has an atomic mass of 40.078 amu. Calcium is a soft grey alkaline earth metal, and is the fifth most abundant element by mass in the earth's Crust ....
-activated plasma membrane-bound enzyme
Enzyme

Enzymes are biomolecules that catalysis chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules at the beginning of the process are called Substrate , and the enzyme converts them into different molecules, the products....
 (magnesium can also activate it) that catalyses the hydrolysis
Hydrolysis

Hydrolysis is a chemical reaction during which one or more water are split into hydrogen and hydroxide ions which may go on to participate in further reactions....
 of ATP
Adenosine triphosphate

This article is about the chemical used by cells as an energy carrier. For other uses, see ATP .Adenosine-5'-triphosphate is a multifunctional nucleotide, and plays an important role in cell biology as a coenzyme that is the "molecule unit of currency" of intracellular energy transfer....
 to yield AMP
Adenosine monophosphate

Adenosine monophosphate , also known as 5'-adenylic acid, is a nucleotide that is found in RNA. It is an ester of phosphoric acid and the nucleoside adenosine....
 and inorganic phosphate. Two isoenzymes are found in commercial preparations from S. tuberosum. One with a higher ratio of substrate selectivity for ATP:ADP (approx 10) and another with no selectivity (ratio 1).

It can also act on ADP
Adenosine diphosphate

Adenosine diphosphate, abbreviated ADP, is a nucleotide. It is an ester of pyrophosphoric acid with the nucleoside adenosine. ADP consists of the pyrophosphate Functional group, the pentose sugar ribose, and the nucleobase adenine....
 and other nucleoside
Nucleoside

Nucleosides are glycosylamines consisting of a nucleobase bound to a ribose or deoxyribose sugar. Examples of these include cytidine, uridine, adenosine, guanosine, thymidine and inosine....
 triphosphates and diphosphates with the general reaction being NTP -> NDP + Pi -> NMP + 2Pi.

It is the enzyme
Enzyme

Enzymes are biomolecules that catalysis chemical reactions. Almost all enzymes are proteins. In enzymatic reactions, the molecules at the beginning of the process are called Substrate , and the enzyme converts them into different molecules, the products....
 released by female mosquito
Mosquito

Mosquitoes are common flying insects in the family Culicidae that are found around the world. There are about 3,500 species. They have a pair of scaled wings, a pair of halteres, a slender body, and six long legs....
es to prevent blood clots when they suck blood.

The activity of apyrase at pH 7.5 in a Tris Buffer is approximately 80% of the activity at pH 6.5.

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