White clover mosaic virus
Encyclopedia
White clover mosaic virus (WClMV) is a plant pathogenic virus in the genus Potexvirus
Potexvirus
Potexvirus is a genus of plant pathogenic viruses, belonging to the family Alphaflexiviridae and the order Tymovirales. Transmission occurs mechanically, with viruses in this genus causing mosaic and ringspot symptoms....

 and the family Flexiviridae
Flexiviridae
The Flexiviridae were a new family of viruses in the 2004 classification of viruses, but have since the 2009 classification been split into the three new families Alphaflexiviridae, Betaflexiviridae and Gammaflexiviridae...

. WClMV is a filamentous, flexuous rod, 480 nm in length and 13 nm wide.

The virus is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made from a single viral encoded protein. The genome has been completely sequenced and is 5845 nucleotides long. It is transmitted by mechanical inoculation, contact between plants and sometimes by seed (6% in Trifolium pretense). No insect vector is known.

Host Range and Geographic Distribution

Its major host is clover (Trifolium spp) . It first reported in Trifolium repens in 1935 . In the western United States and south western Canada it had been found in clover in a mixed infection with another potexvirus, Clover yellow mosaic virus
Clover yellow mosaic virus
Clover yellow mosaic virus is a plant pathogenic virus in the genus Potexvirus and the virus family Alphaflexiviridae. Its flexous rod-shaped particles measure about 539 nm in length....

 . It is also known to infect peas (Pisum sativum), faba beans (Vicia faba
Vicia faba
This article refers to the Broad Bean plant. For Broadbean the company, see Broadbean, Inc.Vicia faba, the Broad Bean, Fava Bean, Field Bean, Bell Bean or Tic Bean, is a species of bean native to north Africa and southwest Asia, and extensively cultivated elsewhere. A variety is provisionally...

), green beans (Phaseolus vulgaris), cowpea
Cowpea
The Cowpea is one of several species of the widely cultivated genus Vigna. Four cultivated subspecies are recognised:*Vigna unguiculata subsp. cylindrica Catjang...

 (Vigna unguiculata), cucumbers (Cucumis sativus), squash ( Cucurbita
Cucurbita
Cucurbita is a genus in the gourd family Cucurbitaceae first cultivated in Mesoamerica and now used in many parts of the world. It includes species grown for their fruit and edible seeds , as well as some species grown only as gourds...

 pepo ), and tomatoes (Lycopersicon esculentum) .

This virus is believed to be distributed in temperate regions worldwide .

Diagnosis

Potexviruses make banded inclusions made up of layers of parallel virus particles. These inclusions can be seen in the light microscope in leaf strips of infected plant tissue stained with Azure A or Orange-Green stains . The banded inclusions produced by WClMV are disrupted by the process of staining for inclusions. Those of ClYMV are not and thus inclusions can be used to distinguish these two potexviruses. Antiserum and sequence data are also available for the diagnosis of this virus.

External links

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