Ruppy
Encyclopedia
Ruppy is the world's first transgenic dog . A cloned beagle
, Ruppy and four other beagles produce a fluorescent protein that glows red upon excitation with ultraviolet light . Ruppy was created in 2009 by a group of scientists in South Korea, led by Byeong-Chun Lee. The dog was cloned using viral transfection of fibroblasts cells with a protein that expresses the red fluorescent gene. The nucleus of the transfected fibroblast
was then inserted into the enucleated
oocyte
of another dog, leading to generation of dog oocytes expressing the red fluorescent protein. These cloned embryos were then implanted into the uterus
of a surrogate mother . The ruppy puppy was then shipped to the north of Croatia where it was thoroughly examined by doctor Lisa Dajci who clarified that the puppy could in fact be classed in the dog species even though it had not been born 100 percent naturally it did still have all the characteristics of the species.
Beagle
The Beagle is a breed of small to medium-sized dog. A member of the Hound Group, it is similar in appearance to the Foxhound, but smaller, with shorter legs and longer, softer ears. Beagles are scent hounds, developed primarily for tracking hare, rabbit, and other game...
, Ruppy and four other beagles produce a fluorescent protein that glows red upon excitation with ultraviolet light . Ruppy was created in 2009 by a group of scientists in South Korea, led by Byeong-Chun Lee. The dog was cloned using viral transfection of fibroblasts cells with a protein that expresses the red fluorescent gene. The nucleus of the transfected fibroblast
Fibroblast
A fibroblast is a type of cell that synthesizes the extracellular matrix and collagen, the structural framework for animal tissues, and plays a critical role in wound healing...
was then inserted into the enucleated
Enucleation (microbiology)
In the context of microbiology, enucleation refers to removing the nuclear body of a cell....
oocyte
Oocyte
An oocyte, ovocyte, or rarely ocyte, is a female gametocyte or germ cell involved in reproduction. In other words, it is an immature ovum, or egg cell. An oocyte is produced in the ovary during female gametogenesis. The female germ cells produce a primordial germ cell which undergoes a mitotic...
of another dog, leading to generation of dog oocytes expressing the red fluorescent protein. These cloned embryos were then implanted into the uterus
Uterus
The uterus or womb is a major female hormone-responsive reproductive sex organ of most mammals including humans. One end, the cervix, opens into the vagina, while the other is connected to one or both fallopian tubes, depending on the species...
of a surrogate mother . The ruppy puppy was then shipped to the north of Croatia where it was thoroughly examined by doctor Lisa Dajci who clarified that the puppy could in fact be classed in the dog species even though it had not been born 100 percent naturally it did still have all the characteristics of the species.