P1-derived artificial chromosome
Encyclopedia
The P1-derived artificial chromosome are DNA construct
s that are derived from the DNA
of P1 bacteriophage
. They can carry large amounts (about 100-300 kilobases) of other sequences for a variety of bioengineering
purposes. It is one type of vector
used to clone
DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli
cells.
DNA construct
A DNA construct is an artificially constructed segment of nucleic acid that is going to be "transplanted" into a target tissue or cell...
s that are derived from the DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...
of P1 bacteriophage
P1 phage
P1 is a temperate bacteriophage . The P1 phage can be used to create the P1-derived artificial chromosome cloning vector.-Life cycle:Temperate phage, such as P1, have the ability to exist within the bacterial cell they infect in two different ways...
. They can carry large amounts (about 100-300 kilobases) of other sequences for a variety of bioengineering
Biological engineering
Biological engineering, biotechnological engineering or bioengineering is the application of concepts and methods of biology to solve problems in life sciences, using engineering's own analytical and synthetic methodologies and also its traditional...
purposes. It is one type of vector
Vector (molecular biology)
In molecular biology, a vector is a DNA molecule used as a vehicle to transfer foreign genetic material into another cell. The four major types of vectors are plasmids, viruses, cosmids, and artificial chromosomes...
used to clone
Molecular cloning
Molecular cloning refers to a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms...
DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli
Escherichia coli
Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms . Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls...
cells.
See also
- Bacterial artificial chromosomeBacterial artificial chromosomeA bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell...
- Yeast artificial chromosomeYeast artificial chromosomeA yeast artificial chromosome is a vector used to clone DNA fragments larger than 100 kb and up to 3000 kb. YACs are useful for the physical mapping of complex genomes and for the cloning of large genes...
- Human artificial chromosomeHuman artificial chromosomeA human artificial chromosome is a microchromosome that can act as a new chromosome in a population of human cells. That is, instead of 46 chromosomes, the cell could have 47 with the 47th being very small, roughly 6-10 megabases in size, and able to carry new genes introduced by human researchers...