ORC6L
Encyclopedia
Origin recognition complex subunit 6 is a protein
that in humans is encoded by the ORC6L gene
.
(ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication
in eukaryotic
cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6
and Mcm
proteins.
, -3L
, -4L
and -5L
. Gene silencing studies with small interfering RNA
demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation
with cytokinesis
.
with MCM5
, ORC2L
, Replication protein A1, ORC4L
, DBF4
, ORC3L
, CDC45-related protein
, MCM4
and Cell division cycle 7-related protein kinase
.
Protein
Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form, facilitating a biological function. A polypeptide is a single linear polymer chain of amino acids bonded together by peptide bonds between the carboxyl and amino groups of...
that in humans is encoded by the ORC6L gene
Gene
A gene is a molecular unit of heredity of a living organism. It is a name given to some stretches of DNA and RNA that code for a type of protein or for an RNA chain that has a function in the organism. Living beings depend on genes, as they specify all proteins and functional RNA chains...
.
Background
The origin recognition complexOrigin Recognition Complex
ORC or Origin Recognition Complex is a multi-subunit DNA binding complex that binds in all eukaryotes in an ATP-dependent manner to origins of replication....
(ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication
DNA replication
DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for biological inheritance. The process starts with one double-stranded DNA molecule and produces two identical copies of the molecule...
in eukaryotic
Eukaryote
A eukaryote is an organism whose cells contain complex structures enclosed within membranes. Eukaryotes may more formally be referred to as the taxon Eukarya or Eukaryota. The defining membrane-bound structure that sets eukaryotic cells apart from prokaryotic cells is the nucleus, or nuclear...
cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6
Cdc6
Cdc6, or Cell Division Cycle 6, is a protein in eukaryotic cells that is studied in the budding yeast Saccharomyces cerevisiae. It is an essential regulator of DNA replication and plays important roles in the activation and maintenance of the checkpoint mechanisms in the cell cycle that coordinate...
and Mcm
Mini Chromosome Maintenance
Mini Chromosome Maintenance complex, or Minichromosome Maintenance protein complex or mini-chromosome maintenance 2-7 helicase complex has a role in both the initiation and the elongation phases of eukaryotic DNA replication, specifically the formation and elongation of the replication fork...
proteins.
Function
The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and ORC1L are loosely associated with the core complex consisting of ORC2LORC2L
Origin recognition complex subunit 2 is a protein that in humans is encoded by the ORC2L gene.-Interactions:ORC2L has been shown to interact with ORC1L, Replication protein A1, ORC4L, ORC5L, ORC3L, MCM4, CDC6, MCM5, MCM6, MCM7, MCM10, DBF4, MCM2 and ORC6L.-Further reading:...
, -3L
ORC3L
Origin recognition complex subunit 3 is a protein that in humans is encoded by the ORC3L gene.-Interactions:ORC3L has been shown to interact with ORC2L, MCM7, ORC4L, ORC5L, MCM4 and ORC6L.-Further reading:...
, -4L
ORC4L
Origin recognition complex subunit 4 is a protein that in humans is encoded by the ORC4L gene.-Interactions:ORC4L has been shown to interact with ORC1L, MCM6, ORC2L, ORC5L, ORC3L, MCM2, MCM4, MCM3 and ORC6L.-Further reading:...
and -5L
ORC5L
Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5L gene.-Interactions:ORC5L has been shown to interact with ORC1L, ORC2L, MCM7, ORC4L, ORC3L, MCM2, MCM4 and MCM3.-Further reading:...
. Gene silencing studies with small interfering RNA
Small interfering RNA
Small interfering RNA , sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA molecules, 20-25 nucleotides in length, that play a variety of roles in biology. The most notable role of siRNA is its involvement in the RNA interference pathway, where it...
demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation
Chromosome segregation
Chromosome segregation is a step in cell reproduction or division, where chromosomes pair off with their similar homologous chromosome. In mitosis, a complete copy of each one is made. In meiosis, one chromosome from each pair migrates to opposite ends of the cell and the genes are split to make a...
with cytokinesis
Cytokinesis
Cytokinesis is the process in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the late stages of mitosis, and sometimes meiosis, splitting a binucleate cell in two, to ensure that chromosome number is maintained from one generation...
.
Interactions
ORC6L has been shown to interactProtein-protein interaction
Protein–protein interactions occur when two or more proteins bind together, often to carry out their biological function. Many of the most important molecular processes in the cell such as DNA replication are carried out by large molecular machines that are built from a large number of protein...
with MCM5
MCM5
DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene.-Interactions:MCM5 has been shown to interact with ORC2L, MCM7, STAT1, MCM2, Cell division cycle 7-related protein kinase, MCM3 and ORC6L.-Further reading:...
, ORC2L
ORC2L
Origin recognition complex subunit 2 is a protein that in humans is encoded by the ORC2L gene.-Interactions:ORC2L has been shown to interact with ORC1L, Replication protein A1, ORC4L, ORC5L, ORC3L, MCM4, CDC6, MCM5, MCM6, MCM7, MCM10, DBF4, MCM2 and ORC6L.-Further reading:...
, Replication protein A1, ORC4L
ORC4L
Origin recognition complex subunit 4 is a protein that in humans is encoded by the ORC4L gene.-Interactions:ORC4L has been shown to interact with ORC1L, MCM6, ORC2L, ORC5L, ORC3L, MCM2, MCM4, MCM3 and ORC6L.-Further reading:...
, DBF4
DBF4
Protein DBF4 homolog A is a protein that in humans is encoded by the DBF4 gene.-Interactions:DBF4 has been shown to interact with ORC2L, MCM7, Cell division cycle 7-related protein kinase, MCM3 and ORC6L.-Further reading:...
, ORC3L
ORC3L
Origin recognition complex subunit 3 is a protein that in humans is encoded by the ORC3L gene.-Interactions:ORC3L has been shown to interact with ORC2L, MCM7, ORC4L, ORC5L, MCM4 and ORC6L.-Further reading:...
, CDC45-related protein
CDC45-related protein
CDC45 is a protein that in humans is encoded by the CDC45L gene.-Interactions:CDC45-related protein has been shown to interact with ORC1L, MCM6, MCM7, MCM3 and ORC6L.-Further reading:...
, MCM4
MCM4
DNA replication licensing factor MCM4 is a protein that in humans is encoded by the MCM4 gene.-Interactions:MCM4 has been shown to interact with ORC1L, ORC2L, Replication protein A1, ORC4L, ORC5L, ORC3L, MCM6, MCM7, MCM2, Cell division cycle 7-related protein kinase and ORC6L.-Further reading:...
and Cell division cycle 7-related protein kinase
Cell division cycle 7-related protein kinase
Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene.- General Information About CDC7 :Cell division cycle 7 is a gene that codes for the protein Cdc7 kinase...
.