Inner root sheath
Encyclopedia
The inner root sheath of the hair follicle is located between the outer root sheath
Outer root sheath
The outer root sheath of the hair follicle encloses the inner root sheath and hair shaft.It is continuous with the basal layer of the interfollicular epidermis ....

 and the hair shaft. It is made of three layers: Henle's layer
Henle's layer
Henle's layer is the third layer of the inner root sheath of the hair, consisting of a single layer of cubical cells with clear flattened nuclei....

, Huxley's layer
Huxley's layer
The second layer of the inner root sheath of the hair consists of one or two layers of horny, flattened, nucleated cells, known as Huxley's layer....

, and the cuticle
Cuticle
A cuticle , or cuticula, is a term used for any of a variety of tough but flexible, non-mineral outer coverings of an organism, or parts of an organism, that provide protection. Various types of "cuticles" are non-homologous; differing in their origin, structure, function, and chemical composition...

.

Henle's layer is the outermost layer of the inner root sheath, consisting of a single row of cubical
Cuboidal epithelia
Cuboidal epithelia are epithelial cells having a cube-like shape; that is, their width is approximately equal to their height. They may exist in single layers or multiple layers depending on their location in the body....

 transparent cells that do not contain nuclei
Cell nucleus
In cell biology, the nucleus is a membrane-enclosed organelle found in eukaryotic cells. It contains most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. The genes within these...

. Huxley's layer is in the middle, made up of approximately two rows of flattened cells with granular protoplasm
Protoplasm
Protoplasm is the living contents of a cell that is surrounded by a plasma membrane. It is a general term of the Cytoplasm . Protoplasm is composed of a mixture of small molecules such as ions, amino acids, monosaccharides and water, and macromolecules such as nucleic acids, proteins, lipids and...

. The cuticle of the root sheath is in the center, continuous with the outermost layer of the hair fiber.
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