DEAE-Sepharose
Encyclopedia
DEAE-Sepharose is a tradename for the anion-exchange
reactive group, diethylaminoethyl
(DEAE) covalently linked to Sepharose
(a polysaccharide
polymer
).
Ion exchange
Ion exchange is an exchange of ions between two electrolytes or between an electrolyte solution and a complex. In most cases the term is used to denote the processes of purification, separation, and decontamination of aqueous and other ion-containing solutions with solid polymeric or mineralic 'ion...
reactive group, diethylaminoethyl
Triethylamine
Triethylamine is the chemical compound with the formula N3, commonly abbreviated Et3N. It is also abbreviated TEA, yet this abbreviation must be used carefully to avoid confusion with triethanolamine, for which TEA is also a common abbreviation....
(DEAE) covalently linked to Sepharose
Sepharose
Sepharose is a tradename for a crosslinked, beaded-form of a polysaccharide polymer material extracted from seaweed. It is crosslinked through lysine side chains. Iodoacetyl functional groups can be added to selectively bind cysteine side chains and this method is often used to immobilize peptides....
(a polysaccharide
Polysaccharide
Polysaccharides are long carbohydrate molecules, of repeated monomer units joined together by glycosidic bonds. They range in structure from linear to highly branched. Polysaccharides are often quite heterogeneous, containing slight modifications of the repeating unit. Depending on the structure,...
polymer
Polymer
A polymer is a large molecule composed of repeating structural units. These subunits are typically connected by covalent chemical bonds...
).