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Centrifuge

 
Centrifuge

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Centrifuge



 
 
A centrifuge is a piece of equipment, generally driven by a motor, that puts an object in rotation around a fixed axis
Rotation around a fixed axis

Rotation around a fixed axis is a special case of rotational motion. It does not involve rotation around more than one axis, and cannot describe such phenomena as wobbling or precession....
, applying a force perpendicular to the axis. The centrifuge works using the sedimentation principle
Sedimentation

Sedimentation describes the motion of molecules in solutions or particle s in suspension in response to an external force such as gravitation, centrifugal force or electromagnetism....
, where the centripetal acceleration is used to evenly distribute substances (usually present in a solution for small scale applications) of greater and lesser density.






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Tabletop Centrifuge
A centrifuge is a piece of equipment, generally driven by a motor, that puts an object in rotation around a fixed axis
Rotation around a fixed axis

Rotation around a fixed axis is a special case of rotational motion. It does not involve rotation around more than one axis, and cannot describe such phenomena as wobbling or precession....
, applying a force perpendicular to the axis. The centrifuge works using the sedimentation principle
Sedimentation

Sedimentation describes the motion of molecules in solutions or particle s in suspension in response to an external force such as gravitation, centrifugal force or electromagnetism....
, where the centripetal acceleration is used to evenly distribute substances (usually present in a solution for small scale applications) of greater and lesser density. There are many different kinds of centrifuges, including those for very specialized purposes. It can be used for viable counts, when shaking the culture e.g. yeast
Yeast

Yeasts are eukaryote microorganisms classified in the Kingdom fungus, with about 1,500 species currently described; they dominate fungal diversity in the oceans....
, out of suspension.

Theory


Protocols for centrifugation typically specify the amount of acceleration
Acceleration

File:Acceleration.JPGFile:Acceleration components.JPGIn physics, and more specifically kinematics, acceleration is the change in velocity over time....
 to be applied to the sample, rather than specifying a rotational speed
Rotational speed

Rotational speed indicates, for example, how fast a motor is running. Rotational speed is equivalent to angular speed, but with different units....
 such as revolutions per minute
Revolutions per minute

Revolutions per minute is a units of measurement of frequency: the number of Turn completed in one minute around a rotation around a fixed axis....
. The acceleration is often quoted in multiples of g, the standard acceleration due to gravity at the Earth's surface. This distinction is important because two rotors with different diameters running at the same rotational speed will subject samples to different accelerations.

The acceleration can be calculated as the product of the radius
RADIUS

Remote Authentication Dial In User Service is a networking protocol that provides centralized access, authorization and accounting management for people or computers to connect and use a network service....
 and the square of the angular velocity
Angular velocity

In physics, the angular velocity is a vector quantity which specifies the angular speed, and axis about which an object is rotating. The SI unit of angular velocity is radians per second, although it may be measured in other units such as degrees per second, revolutions per second, degrees per hour, etc....
.

History and predecessors

English military engineer Benjamin Robins
Benjamin Robins

Benjamin Robins was an England science, mathematics, and engineer.Robins, Benjamin was a Newtonian mathematician, military engineer for the British East India Company, and pioneering ballistics researcher who invented the ballistic pendulum and authored New Principles of Gunnery ?the first significant application of Newtonian science to e...
 (1707-1751) invented a whirling arm apparatus to determine drag
Drag (physics)

The term drag is widely used in Physics and Engineering and is central to the field of fluid dynamics. "Drag" refers to forces that oppose the motion of a solid object through a fluid ....
. In 1864, Antonin Prandtl invented the first dairy centrifuge in order to separate cream from milk. In 1879, Gustaf de Laval
Gustaf de Laval

Karl Gustaf Patrik de Laval was a Sweden engineer and inventor who made important contributions to the design of steam turbines and dairy machinery....
 demonstrated the first continuous centrifugal separator, making its commercial application feasible.

Types

There are at least five types of centrifuge:
  • preparative centrifuge
  • analytical centrifuge
  • angle fixed centrifuge
  • swing head centrifuge
  • haemacrotic centrifuge]]


Industrial centrifuges may otherwise be classified according to the type of separation of the high density fraction from the low density one :
  • Screen centrifuges, where the centrifugal acceleration allows the liquid to pass through a screen
    Screen

    Screen may refer to:...
     of some sort, through which the solids cannot go (due to granulometry
    Granulometry

    Granulometry is the measurement of the size distribution in a collection of grains.* Granulometry : Granulometry computation using the opening operation....
     larger than the screen gap or due to agglomeration). Common types are :
    • Pusher centrifuges
    • Peeler centrifuges
  • Decanter centrifuges, in which there is no physical separation between the solid and liquid phase, rather an accelerated settling
    Settling

    Settling is the process by which particulates settle to the bottom of a liquid and form a sediment. ]Particles that experience a force, either due to gravity or due to centrifugal motion will tend to move in a uniform manner in the direction exerted by that force....
     due to centrifugal acceleration. Common types are :
    • Solid bowl centrifuges
    • Conical plate centrifuges


Uses


Isolating suspensions

Simple centrifuges are used in chemistry, biology, and biochemistry for isolating and separating suspensions. They vary widely in speed and capacity. They usually comprise a rotor containing two, four, six, or many more numbered wells within which the samples containing centrifuge tips may be placed.

Isotope separation

Other centrifuges, the first being the Zippe-type centrifuge
Zippe-type centrifuge

The Zippe-type centrifuge is a particular design of gas centrifuge. It was developed in the Soviet Union by a team of 60 German scientists working in detention, captured after World War II....
, separate isotope
Isotope

Isotopes are any of the different types of atoms of the same chemical element, each having a different atomic mass . Isotopes of an element have atomic nucleus with the same number of protons but different numbers of neutron....
s, and these kinds of centrifuges are in use in nuclear power
Nuclear power

Nuclear power is any nuclear technology designed to extract usable energy from atomic nucleus via controlled nuclear reactions. The only method in use today is through nuclear fission, though other methods might one day include nuclear fusion and radioactive decay ....
 and nuclear weapon
Nuclear weapon

A nuclear weapon is an explosive device that derives its destructive force from nuclear reactions, either nuclear fission or a combination of fission and nuclear fusion....
 programs.

Gas centrifuge
Gas centrifuge

A gas centrifuge is a separating machine specifically developed to separate Uranium-235 from Uranium-238. The gas centrifuge relies on the principles of centripetal force accelerating molecules based upon mass....
s are used in uranium enrichment. The heavier isotope
Isotope

Isotopes are any of the different types of atoms of the same chemical element, each having a different atomic mass . Isotopes of an element have atomic nucleus with the same number of protons but different numbers of neutron....
 of uranium (uranium-238
Uranium-238

Uranium-238 , is the most common Isotopes of uranium of uranium found in nature. When hit by a neutron, it becomes uranium-239 , an unstable isotope which radioactive decay into neptunium-239 , which then itself decays, with a half-life of 2.355 days, into plutonium-239 ....
) in the uranium hexafluoride
Uranium hexafluoride

Uranium hexafluoride , referred to as "hex" in the nuclear industry, is a compound used in the uranium Isotope separation#Centrifugal Force process that produces fuel for nuclear reactors and nuclear weapons....
 gas tend to concentrate at the walls of the centrifuge as it spins, while the desired uranium-235
Uranium-235

Uranium-235 is an Isotopes of uranium that differs from the element's other common isotope, uranium-238, by its ability to cause a rapidly expanding nuclear fission chain reaction, i.e., it is fissile....
 isotope is extracted and concentrated with a scoop selectively placed inside the centrifuge. It takes many thousands of centrifuges to enrich uranium enough for use in a nuclear reactor
Nuclear reactor

A nuclear reactor is a device in which nuclear chain reactions are initiated, controlled, and sustained at a steady rate, as opposed to a nuclear bomb, in which the chain reaction occurs in a fraction of a second and is uncontrolled causing an explosion....
 (around 3.5% enrichment), and many thousands more to enrich it to weapons-grade
Weapons-grade

A weapons-grade substance is one that is pure enough to be used to make a weapon or has properties that make it suitable for weapons use. Weapons-grade plutonium and uranium are the most common examples, but it is also be used to refer to chemical warfare and biological warfare....
 (around 90% enrichment) for use in nuclear weapons.

Aeronautics and astronautics

Human centrifuges are exceptionally large centrifuges that test the reactions and tolerance of pilots
Aviator

An aviator is a person who flies aircraft for pleasure or as a profession.The feminine word aviatrix is sometimes used and is the correct term to refer to all women pilots....
 and astronaut
Astronaut

An astronaut or cosmonaut is a person trained by a List of human spaceflight programs to command, pilot, or serve as a crew member of a spacecraft....
s to acceleration above those experienced in the Earth's gravity.

The US Air Force at Holloman Air Force Base
Holloman Air Force Base

Holloman Air Force Base is a United States Air Force base located six miles southwest of the central business district of Alamogordo, New Mexico, a city in Otero County, New Mexico, New Mexico, United States....
, NM operates a human centrifuge. The centrifuge at Holloman AFB is operated by the aerospace physiology
Aerospace physiology

Aerospace physiology refers to effects on the human body caused by characteristics of the aerospace environment.This subject includes, but is not limited to, the following topics:...
 department for the purpose of training and evaluating prospective fighter pilots for high-g flight in Air Force fighter aircraft. It is important to note that the centrifuge at Holloman AFB is unrealistic in that it is far more difficult for a pilot to tolerate the high-g environment in the centrifuge than in a real fighter aircraft. This well-known fact is based on countless accounts from experienced operational fighter pilots.

The use of large centrifuges to simulate a feeling of gravity has been proposed for future long-duration space missions. Exposure to this simulated gravity would prevent or reduce the bone decalcification
Bone decalcification

Bone decalcification is the removal of calcium ions from the bone through histological process thereby making the bone flexible and easy for pathological investigation....
 and muscle atrophy
Muscle atrophy

Muscle atrophy is defined as a decrease in the mass of the muscle; it can be a partial or complete wasting away of muscle. When a muscle atrophies, it becomes weaker, since the ability to exert force is related to mass; muscle atrophy is a co-morbidity of several common diseases, including cancer, AIDS, congestive heart disease, COPD , renal...
 that affect individuals exposed to long periods of freefall. An example of this can be seen in the film 2001: A Space Odyssey
2001: A Space Odyssey (film)

2001: A Space Odyssey is a 1968 in film science fiction film directed by Stanley Kubrick, written by Kubrick and Arthur C. Clarke. The film deals with thematic elements of human evolution, technology, artificial intelligence, and extraterrestrial life, and is notable for its scientific realism, pioneering special effects, ambiguous and of...
, while the James Bond
James Bond

James Bond 007 is a fictional character created in 1953 by writer Ian Fleming, who featured him in twelve novels and two short story collections....
 film Moonraker
Moonraker (film)

Moonraker is the eleventh spy film in the James Bond James Bond , and the fourth to star Roger Moore as the fictional character Secret Intelligence Service agent James Bond ....
 shows an astronaut-training centrifuge in action on earth (albeit sabotaged with murderous intent).

Earthquake and blast simulation

The geotechnical centrifuge is used for simulating blasts and earthquake phenomena. For a discussion of their design, see .

Commercial applications

  • Standalone centrifuges for drying (hand-washed) clothes - usually with a water outlet.
  • Centrifuges are used in the attraction Mission: SPACE
    Mission: SPACE

    Mission: SPACE is a motion simulator thrill ride at Epcot. It simulates what an astronaut might experience aboard a spacecraft on a mission to Mars, from the higher g-force of blastoff to the speculative suspended animation....
    , located at Epcot
    Epcot

    Epcot is a theme park at the Walt Disney World Resort. The park is dedicated to international culture and technological innovation. The second park built at the resort, it opened on October 1, 1982 and was named EPCOT Center until 1994....
     in Walt Disney World, which propels riders using a combination of a centrifuge and a motion simulator
    Motion simulator

    A motion simulator or motion platform is a mechanism that encapsulates riders and creates the effect/feelings of being in a moving object....
     to simulate the feeling of going into space
    Space

    Space is the boundless, three-dimensional extent in which Physical body and events occur and have relative position and direction. Physical space is often conceived in three linear dimensions, although modern physics usually consider it, with time, to be part of the boundless four-dimensional continuum known as spacetime....
    .
  • In soil mechanics
    Soil mechanics

    Soil mechanics is a discipline that applies principles of engineering mechanics, e.g. kinematics, dynamics, fluid mechanics, and mechanics of material, to predict the mechanical behavior of soils....
    , centrifuges utilize centrifugal acceleration to match soil stresses in a scale model to those found in reality.
  • Large industrial centrifuges are commonly used in water
    Water

    Water is a common chemical substance that is essential for the survival of all known forms of life. In typical usage, water refers only to its liquid form or States of matter, but the substance also has a solid state, ice, and a gaseous state, water vapor or steam....
     and wastewater
    Wastewater

    Wastewater is any water that has been adversely affected in quality by anthropogenic influence. It comprises liquid waste discharged by domestic residences, commercial properties, industry, and/or agriculture and can encompass a wide range of potential contaminants and concentrations....
     treatment to dry sludge
    Sludge

    Sludge is the residual semi-solid material left from industrial, or wastewater Sewage_treatment#Secondary_treatment. When fresh sewage or wastewater is added to a settling Storage tank, approximately 50% of the suspended solid matter will settle out in an hour and a half....
    s. The resulting dry product is often termed cake, and the water leaving a centrifuge after most of the solids have been removed is called centrate.
  • Large industrial centrifuges are also used in the oil industry to remove solids from the drilling fluid
    Drilling fluid

    Drilling a very deep hole is a very costly business. The hole size is kept very small as the drilled depth increases because it is to cased and cemented after wards....
    .
  • Disc-stack centrifuges used by some companies in Oil Sands industry to separate small amounts of water and solids from bitumen before it's sent to Upgrading.


Calculating relative centrifugal force (RCF)


Relative centrifugal force is the measurement of the force applied to a sample within a centrifuge. This can be calculated from the speed (RPM) and the rotational radius (cm) using the following calculation.



where

g = Relative centrifuge force
r = rotational radius (centimetre
Centimetre

A centimetre is a Units of measurement of length in the metric system, equal to one hundredth of a metre, which is the current International System of Units SI base unit of length....
s, cm)


N = rotating speed (revolutions per minute
Revolutions per minute

Revolutions per minute is a units of measurement of frequency: the number of Turn completed in one minute around a rotation around a fixed axis....
, r/min)


Further reading



See also

  • Lamm equation
    Lamm equation

    The Lamm equation describes the sedimentation and diffusion of a solute under ultracentrifuge in traditional Circular sector-shaped cells. The Lamm equation can be written:...
  • Sedimentation
    Sedimentation

    Sedimentation describes the motion of molecules in solutions or particle s in suspension in response to an external force such as gravitation, centrifugal force or electromagnetism....
  • Centrifugal force
    Centrifugal force

    In classical mechanics, centrifugal force is an outward force associated with rotation. Centrifugal force is one of several so-called pseudo-forces , so named because, unlike Fundamental interaction, they do not originate in interactions with other bodies situated in the environment of the particle upon which they act....
  • Centrifugation
    Centrifugation

    Centrifugation is a process that involves the use of the centrifugal force for the separation processs, used in industry and in laboratory settings....
  • Gas centrifuge
    Gas centrifuge

    A gas centrifuge is a separating machine specifically developed to separate Uranium-235 from Uranium-238. The gas centrifuge relies on the principles of centripetal force accelerating molecules based upon mass....
  • Sedimentation coefficient
    Sedimentation coefficient

    The sedimentation coefficient s of a particle is used to characterize its behaviour in sedimentation processes, notably centrifugation. It is defined as the ratio of a particle's sedimentation velocity to the acceleration that is applied to it ....
  • Clearing factor
    Clearing factor

    In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed....


External links

  • in the Virtual Laboratory of the Max Planck Institute for the History of Science
    Max Planck Institute for the History of Science

    The Max Planck Institute for the History of Science in Berlin was established in March 1994. Its research is primarily devoted to a theoretically oriented history of science, principally of the natural sciences, but with methodological perspectives drawn from the cognitive sciences and from cultural history....