Artificial transcription factor
Encyclopedia
An artificial transcription factor is an example of a chimeral protein, designed to target and modulate gene transcription.
They are generally composed of a DNA-binding domain (specific to a certain sequence) coupled to a modulatory domain (which acts upon other transcription factors) in order to alter the expression of a particular gene. It is also possible to downregulate expression of a gene by targeting the 5' untranslated region with a DNA-binding domain that lacks a regulatory domain; this will reduce transcription simply by blocking RNA polymerase
progression along the DNA template.
They are generally composed of a DNA-binding domain (specific to a certain sequence) coupled to a modulatory domain (which acts upon other transcription factors) in order to alter the expression of a particular gene. It is also possible to downregulate expression of a gene by targeting the 5' untranslated region with a DNA-binding domain that lacks a regulatory domain; this will reduce transcription simply by blocking RNA polymerase
RNA polymerase
RNA polymerase is an enzyme that produces RNA. In cells, RNAP is needed for constructing RNA chains from DNA genes as templates, a process called transcription. RNA polymerase enzymes are essential to life and are found in all organisms and many viruses...
progression along the DNA template.
See also
- Therapeutic gene modulationTherapeutic gene modulationTherapeutic gene modulation refers the practice of altering the expression of a gene at one of various stages, with a view to alleviate some form of ailment...
- Zinc fingerZinc fingerZinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules...
- Zinc finger protein transcription factorZinc finger protein transcription factorZinc finger protein transcription factors or ZFP-TFs, consisting of activators and repressors are transcription factors composed of a zinc finger protein domain and any of a variety of transcription-factor effector-domains which exert their modulatory effect in the vicinity of any sequence to...
- Chimera (protein)
- Protein engineeringProtein engineeringProtein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles....