Treponema pallidum particle agglutination assay
Encyclopedia
The Syphilis TPHA test is an indirect hemagglutination assay used for the detection and titration of antibodies against the causative agent of syphilis
,Treponema pallidum subspecies pallidum.
In the test, red blood cells (erythrocytes) are sensitized with antigens from T. pallidum pallidum. The cells then aggregate on the surface of a test dish if exposed to the serum of a patient with syphilis.
Antibodies against other treponemal organisms, such as the T. pallidum subspecies endemicum, pertenue, or carateum, can cause false positive results. A variety of methods exist to adsorb these antibodies from the test sample before hemagglutination.
Syphilis
Syphilis is a sexually transmitted infection caused by the spirochete bacterium Treponema pallidum subspecies pallidum. The primary route of transmission is through sexual contact; however, it may also be transmitted from mother to fetus during pregnancy or at birth, resulting in congenital syphilis...
,Treponema pallidum subspecies pallidum.
In the test, red blood cells (erythrocytes) are sensitized with antigens from T. pallidum pallidum. The cells then aggregate on the surface of a test dish if exposed to the serum of a patient with syphilis.
Antibodies against other treponemal organisms, such as the T. pallidum subspecies endemicum, pertenue, or carateum, can cause false positive results. A variety of methods exist to adsorb these antibodies from the test sample before hemagglutination.