Saccharomyces kluyveri
is a plant pathogen.
Habitat
S. kluyveris habitat has not been as extensively characterized as that of yeast species such as S. cerevisiae or Candida albicans; only about 30 isolates have been described. However, the species appears to be wide spread in the environment. It has been isolated from Drosophila species in North America, soil in Europe, and various tree species in India and North America. Little data has been published on S. kluyveri's habitat since it was first described in 1956, but there is a report of S. kluyveri being isolated from the tissues of an HIV infected patient.
Biology
Saccharomyces kluyveri is a budding yeast related to Saccharomyces cerevisiae, or baker's yeast, the model organism intensively used in biochemistry, genetics and cell biology. S. cerevisiae and S. kluyveri have several fundamental differences that warrant genomic comparisons. First, like most cell types, S. kluyveri resorts to fermentation (degrading sugars in the absence of oxygen) only when oxygen is limiting. S. cerevisiae, on the other hand, prefers to carry out fermentation even in the presence of oxygen. This means that S. kluyveri makes a more efficient use of glucose for energy production. Therefore, S. kluyveri provides a contrasting model to one of the most unique features of S. cerevisiae. Second, S. kluyveri has a simpler genome organization than S. cerevisiae: it appears to have become a species before the whole genome duplication that occurred in the Saccharomyces lineage. As a result, its genome is smaller (about 9.5 million base pairs) than that of S. cerevisiae with fewer duplicated genes. Additionally, S. kluyveri is becoming more widely used as a model organism and for industrial applications, such as the production of proteins, since its biomass yield is greater than that of S. cerevisiae due to more efficient use of glucose.
Sequencing information
The S. kluyveri genome was originally sequenced in 2002 to approximately 3.5X whole genome shotgun (WGS) coverage in collaboration with Mark Johnston in the Department of Genetics. We have now completed an additional 4X coverage of the genome including WGS and 25X clone coverage of fosmid end sequence templates, yielding a total of 7.5X genome coverage. Manual and automated finishing are ongoing, with 10,000 reactions planned. The National Human Genome Research Institute (NHGRI), National Institutes of Health (NIH), provided the funding for the genome sequence.
External links
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