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In chromatography
Chromatography

Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated....
, the retardation factor (also known as retention factor) describes the ratio of time spent in the stationary phase relative to time spent in the mobile phase.

example, if particular substance in an unknown mixture travels 2.5 cm and the solvent front travels 5.0 cm, the retention factor would be 0.5.






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In chromatography
Chromatography

Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated....
, the retardation factor (also known as retention factor) describes the ratio of time spent in the stationary phase relative to time spent in the mobile phase.

Planar chromatography


The retardation factor in planar chromatography, Rf can be mathematically described by the following ratio:

For example, if particular substance in an unknown mixture travels 2.5 cm and the solvent front travels 5.0 cm, the retention factor would be 0.5. An Rf value will always be in the range 0 to 1: if the substance moves at all, it moves along the direction the solvent ("mobile phase") does less, but cannot move further than the solvent does. Rf values are only useful if they are between these two extremes. One can choose a mobile phase with different characteristics (especially polarity) in order to control how far the substance being investigated migrates. An Rf value is characteristic for any given compound (provided that the same stationary and mobile phases are used).

It can provide corroborative evidence as to the identity of a compound. If the identity of a compound is suspected but not yet proven, an authentic sample of the compound, or standard, is spotted and run on a TLC plate side by side (or on top of each other) with the compound in question. Note that this identity check must be performed on a single plate, because it is difficult to duplicate all the factors which influence Rf exactly from experiment to experiment.

Column chromatography

In column chromatography
Column chromatography

Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms....
 the retardation factor, R, is the fraction of the sample in the mobile phase at equilibrium. It is expressed as

where k is the retention factor.

See also

  • Paper chromatography
    Paper chromatography

    Paper chromatography is an analytical chemistry technique for separating and identifying mixtures that are or can be colored, especially pigments....
  • Thin layer chromatography
    Thin layer chromatography

    Thin layer chromatography is a chromatography technique used to separate mixtures. Thin layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with the a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose....