State Route 335 is a north–south state highway

State highway

State highway, state road or state route can refer to one of three related concepts, two of them related to a state or provincial government in a country that is divided into states or provinces :#A...

 in the southern portion of the U.S. state

U.S. state

A U.S. state is any one of the 50 federated states of the United States of America that share sovereignty with the federal government. Because of this shared sovereignty, an American is a citizen both of the federal entity and of his or her state of domicile. Four states use the official title of...

 of Ohio

Ohio

Ohio is a Midwestern state in the United States. The 34th largest state by area in the U.S.,it is the 7th‑most populous with over 11.5 million residents, containing several major American cities and seven metropolitan areas with populations of 500,000 or more.The state's capital is Columbus...

. Its southern terminus is at U.S. Route 52 in the village of Sciotoville, part of Portsmouth

Portsmouth, Ohio

Portsmouth is a city in the U.S. state of Ohio and the county seat of Scioto County. The municipality is located on the northern banks of the Ohio River and east of the Scioto River in Southern Ohio. The population was 20,226 at the 2010 census.-Foundation:...

, and its northern terminus is at State Route 220 in Waverly

Waverly, Ohio

Waverly is a village in and the county seat of Pike County, Ohio, United States. The population was at 4,433 at the 2000 census. The town was formed in 1829, as the construction of the Ohio and Erie Canal along the west bank of the Scioto River brought new growth to the area. In 1861 the county...

 where it’s concurrent with U.S. Route 23 and State Route 104 for about 1/3 of a mile.

Along the way, it intersects with Ohio 139 in Minford

Minford, Ohio

Minford is a census-designated place located on the border of Harrison and Madison Townships in northeastern Scioto County, Ohio, United States, about 14 miles northeast of the county seat of Portsmouth.-History:...

 and Ohio 776 near Stockdale. It crosses Ohio 32 near Beaver.

History

• 1933 – Original route established; originally routed from Minford
  Minford, Ohio
  Minford is a census-designated place located on the border of Harrison and Madison Townships in northeastern Scioto County, Ohio, United States, about 14 miles northeast of the county seat of Portsmouth.-History:...
  
   to Beaver
  Beaver, Ohio
  Beaver is a village in Pike County, Ohio, United States. The population was 464 at the 2000 census.Beaver Eastern Pike serves as the local school district for surrounding areas. Beaver is served by the Eastern Branch of the Garnet A...
  
   along its current alignment.
• 1938 – Extended north to Waverly
  Waverly, Ohio
  Waverly is a village in and the county seat of Pike County, Ohio, United States. The population was at 4,433 at the 2000 census. The town was formed in 1829, as the construction of the Ohio and Erie Canal along the west bank of the Scioto River brought new growth to the area. In 1861 the county...
  
   via a previously unnumbered road.
• 1939 – Extended south to Portsmouth
  Portsmouth, Ohio
  Portsmouth is a city in the U.S. state of Ohio and the county seat of Scioto County. The municipality is located on the northern banks of the Ohio River and east of the Scioto River in Southern Ohio. The population was 20,226 at the 2010 census.-Foundation:...
  
  via a previously unnumbered road.

During its concurency with U.S. 23 and State ROute 104 in Waverly, its signage is backwards, hence where it joins 23, 335 North becomes 335 South, and 335 South becomes North. For example, The signs during the concurrency should read 23/104 North, 335 South, but instead they all say North.