Moving boundary electrophoresis
Encyclopedia
Moving-boundary electrophoresis or free-boundary electrophoresis is electrophoresis
in a free solution. It was developed by Arne Tiselius
in 1937. Tiselius was awarded the 1948 Nobel Prize in chemistry for his work on the separation of colloids through electrophoresis, the motion of charged particles through a stationary liquid under the influence of an electric field.
The apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any mixture of charged components like a protein mixture. On applying voltage, the compounds will migrate to the anode or cathode depending on their charges. The change in the refractive index at the boundary of the separated compounds is detected using Schlieren
optics at both ends of the solution in the cell.
Electrophoresis
Electrophoresis, also called cataphoresis, is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field. This electrokinetic phenomenon was observed for the first time in 1807 by Reuss , who noticed that the application of a constant electric...
in a free solution. It was developed by Arne Tiselius
Arne Tiselius
Arne Wilhelm Kaurin Tiselius was a Swedish biochemist who won the Nobel Prize in Chemistry in 1948.- Biography:Tiselius was born in Stockholm...
in 1937. Tiselius was awarded the 1948 Nobel Prize in chemistry for his work on the separation of colloids through electrophoresis, the motion of charged particles through a stationary liquid under the influence of an electric field.
The apparatus includes a U-shaped cell filled with buffer solution and electrodes immersed at its ends. The sample applied could be any mixture of charged components like a protein mixture. On applying voltage, the compounds will migrate to the anode or cathode depending on their charges. The change in the refractive index at the boundary of the separated compounds is detected using Schlieren
Schlieren
Schlieren are optical inhomogeneities in transparent material not visible to the human eye. Schlieren physics developed out of the need to produce high-quality lenses devoid of these inhomogeneities. These inhomogeneities are localized differences in optical path length that cause light deviation...
optics at both ends of the solution in the cell.
External links
- Arne Wilhelm Kaurin Tiselius — Information on Tiselius compiled from various sources.