In vivo selection of an entire exon

Exon

An exon is a nucleic acid sequence that is represented in the mature form of an RNA molecule either after portions of a precursor RNA have been removed by cis-splicing or when two or more precursor RNA molecules have been ligated by trans-splicing. The mature RNA molecule can be a messenger RNA...

 is a powerful technique used by Singh

Singh

Also see SinhaSingh is a common title, middle name, or surname in Northern India and South India used by sikhs warriors and kings. eg. Man Singh I, Maharana Pratap Singh. It is derived from the Sanskrit word Siṃha meaning "lion and used by Ahir kings of Nepal". It is also used in Sri Lanka by...

 and coworkers to identify novel regulatory elements within an exonic sequence that undergoes alternative splicing. Thanks to in vivo, selection of the entire exon seven the of SMN gene

Gene

A gene is a molecular unit of heredity of a living organism. It is a name given to some stretches of DNA and RNA that code for a type of protein or for an RNA chain that has a function in the organism. Living beings depend on genes, as they specify all proteins and functional RNA chains...

, many regulatory elements have been identified. A knowledge of such elements is crucial for understanding the underlying cause of Spinal Muscular Atrophy

Spinal muscular atrophy

Spinal Muscular Atrophy is a neuromuscular disease characterized by degeneration of motor neurons, resulting in progressive muscular atrophy and weakness. The clinical spectrum of SMA ranges from early infant death to normal adult life with only mild weakness...

, a devastating neuromuscular genetic disease of children and infants.