IRIS (Biosensor)
Encyclopedia
Interferometric Reflectance Imaging Sensor (IRIS) is a system that was formerly known as the Spectral Reflectance Imaging Biosensor (SRIB)
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It can be used a biosensing platform that is capable of high-throughput multiplexing of protein-protein, protein-DNA, and DNA-DNA interactions without the use of any fluorescent labels. The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si/SiO2 substrates. IRIS is capable of quantifying bimolecular mass accumulated on the surface
detection, pathogen detection and bio-defense monitoring, kinetic analysis of biomolecular interactions, and general biomolecular interaction studies for research applications.
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It can be used a biosensing platform that is capable of high-throughput multiplexing of protein-protein, protein-DNA, and DNA-DNA interactions without the use of any fluorescent labels. The sensing surface is prepared by robotic spotting of biological probes that are immobilized on functionalized Si/SiO2 substrates. IRIS is capable of quantifying bimolecular mass accumulated on the surface
Measurement
To perform a measurement, the sample is illuminated with multiple different wavelengths from either a tunable laser or different color LEDs; typically speaking, a relatively narrow bandwidth optical source. The reflection intensity is imaged using a CCD or CMOS camera. By using interferometric techniques, nanometer changes can be detected.Applications
Applications for IRIS include microarray format immunoassays, single nucleotide polymorphism (SNP)detection, pathogen detection and bio-defense monitoring, kinetic analysis of biomolecular interactions, and general biomolecular interaction studies for research applications.