Borate buffered saline
Encyclopedia
Borate Buffered Saline is a buffer used in some biochemical
techniques to maintain the pH
within a relatively narrow range. Borate buffers has an alkaline buffering capacity in the 8 - 10 range.
Boric acid
has a pKa of 9.14 at 25C.
Adjust pH to pH 8.2
The simplest way to prepare a BBS solution is to use BBS tablets. They are formulated to give a ready to use Borate buffered saline solution upon dissolution in 500 ml of deionized water.
Note that concentration of borate and NaCl as well as the pH can vary, and the resulting solution would still be referred to as "Borate buffered saline".
Borate concentration (giving buffering capacity) can vary from 10 mM to 100 mM. As BBS is used to emulate physiological conditions (as in animal or human body), the pH value is slightly alkaline, ranging from 8.0 - 9.0.
NaCl gives the isotonic (mostly used 150 mM NaCl corresponds to physiological conditions: 0.9% NaCl) salt concentration.
Additionally Tween 20 can also be added in order to use it as wash solution in immunoassays or to block.
Biochemistry
Biochemistry, sometimes called biological chemistry, is the study of chemical processes in living organisms, including, but not limited to, living matter. Biochemistry governs all living organisms and living processes...
techniques to maintain the pH
PH
In chemistry, pH is a measure of the acidity or basicity of an aqueous solution. Pure water is said to be neutral, with a pH close to 7.0 at . Solutions with a pH less than 7 are said to be acidic and solutions with a pH greater than 7 are basic or alkaline...
within a relatively narrow range. Borate buffers has an alkaline buffering capacity in the 8 - 10 range.
Boric acid
Boric acid
Boric acid, also called hydrogen borate or boracic acid or orthoboric acid or acidum boricum, is a weak acid of boron often used as an antiseptic, insecticide, flame retardant, as a neutron absorber, and as a precursor of other chemical compounds. It exists in the form of colorless crystals or a...
has a pKa of 9.14 at 25C.
Applications
BBS has many uses because it is isotonic and has a strong bactericidal effect. It can be used to dilute substances and in coating procedures. Additives such as Tween 20 and milk powder can be used to add function as washing buffer or blocking buffer.Contents of BBS
The following is a sample recipe for BBS:- 10 mM Sodium borateBorateBorates are chemical compounds which contain oxoanions of boron in oxidation state +3. The simplest borate ion, BO33−, has a trigonal planar structure. Other borates are made up of trigonal BO3 or tetrahedral BO4 structural units, sharing oxygen atoms...
- 150 mM NaCl
Adjust pH to pH 8.2
The simplest way to prepare a BBS solution is to use BBS tablets. They are formulated to give a ready to use Borate buffered saline solution upon dissolution in 500 ml of deionized water.
Note that concentration of borate and NaCl as well as the pH can vary, and the resulting solution would still be referred to as "Borate buffered saline".
Borate concentration (giving buffering capacity) can vary from 10 mM to 100 mM. As BBS is used to emulate physiological conditions (as in animal or human body), the pH value is slightly alkaline, ranging from 8.0 - 9.0.
NaCl gives the isotonic (mostly used 150 mM NaCl corresponds to physiological conditions: 0.9% NaCl) salt concentration.
Additionally Tween 20 can also be added in order to use it as wash solution in immunoassays or to block.