Acid Citrate Dextrose Solution (sometimes called Anticoagulant Citrate Dextrose Solution) is a solution of
citric acidCitric acid is a weak organic acid. It is a natural preservative/conservative and is also used to add an acidic, or sour, taste to foods and soft drinks...
,
sodium citrateMonosodium citrate, or sodium dihydrogen citrate, is an acid salt with the chemical formula NaH2C6H5O7, or C3H4OH2COONa. Since it has two remaining open spots on the citrate anion, it is used as a relatively strong sequestrant. It is used to prevent platelet clumping in blood samples...
and dextrose in water. It is mainly used as an
anticoagulantAn anticoagulant is a substance that prevents coagulation of blood. A group of pharmaceuticals called anticoagulants can be used in vivo as a medication for thrombotic disorders. Some anticoagulants are used in medical equipment, such as test tubes, blood transfusion bags, and renal dialysis...
to preserve blood specimens required for
tissue typingTissue typing is a procedure in which the tissues of a prospective donor and recipient are tested for compatibility prior to transplantation. An embryo can be tissue typed to ensure that the embryo implanted can be a cord-blood stem cell donor for a sick sibling.One technique of tissue typing,...
, it is also used during procedures such as
plasmapheresisPlasmapheresis is the removal, treatment, and return of blood plasma from blood circulation. It is thus an extracorporeal therapy...
instead of
heparinHeparin , also known as unfractionated heparin, a highly sulfated glycosaminoglycan, is widely used as an injectable anticoagulant, and has the highest negative charge density of any known biological molecule...
. Two different solutions (Solution A and B) are defined by the
United States PharmacopeiaThe United States Pharmacopeia is the official pharmacopeia of the United States, published dually with the National Formulary as the USP-NF. The United States Pharmacopeial Convention is the nonprofit organization that owns the trademark and copyright to the USP-NF and publishes it every year...
.
They have the following properties:
Solution A (per 1000 mL)
Total Citrate (as Citric acidCitric acid is a weak organic acid. It is a natural preservative/conservative and is also used to add an acidic, or sour, taste to foods and soft drinks...
, anhydrous (C6H8O7)) |
20.59 to 22.75g |
| Dextrose (C6H12O6*H2O) |
23.28g to 25.73g |
SodiumSodium is a chemical element with the symbol Na and atomic number 11. It is a soft, silvery-white, highly reactive metal and is a member of the alkali metals; its only stable isotope is 23Na. It is an abundant element that exists in numerous minerals, most commonly as sodium chloride...
(Na) |
4.90g to 5.42g |
Solution B (per 1000 mL)
| Total Citrate (as Citric acid, anhydrous (C6H8O7)) |
12.37 to 13.67g |
| Dextrose (C6H12O6*H2O) |
13.96 to 15.44g |
| Sodium (Na) |
2.94 to 3.25g |
To make use:
| |
A |
B |
| Citric Acid, anhydrous (C6H8O7) |
7.3g |
4.4 |
| Sodium Citrate, dihydrate |
22.0g |
13.2 |
| Dextrose, monohydrate (C6H12O6*H2O) |
24.5g |
14.7 |
WaterWater is a chemical substance with the chemical formula H2O. A water molecule contains one oxygen and two hydrogen atoms connected by covalent bonds. Water is a liquid at ambient conditions, but it often co-exists on Earth with its solid state, ice, and gaseous state . Water also exists in a...
for Injection to make |
1000 mL |
1000 mL |
Dissolve the ingredients and mix. Filter until clear.