I wonder why the peak at 480 nm in uv-vis absorption spectra of ET-Br is used to determine the change in its concentration, why not the peak at 285 nm? Which one is more accurate to use?

Ethidium bromide

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dscience

I wonder why the peak at 480 nm in uv-vis absorption spectra of ET-Br is used to determine the change in its concentration, why not the peak at 285 nm? Which one is more accurate to use?

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