Rosenbach's test
Encyclopedia
Rosenbach's test is a qualitative
Qualitative research
Qualitative research is a method of inquiry employed in many different academic disciplines, traditionally in the social sciences, but also in market research and further contexts. Qualitative researchers aim to gather an in-depth understanding of human behavior and the reasons that govern such...

 medical test
Medical test
A diagnostic test is any kind of medical test performed to aid in the diagnosis or detection of disease. For example:* to diagnose diseases, and preferably sub-classify it regarding, for example, severity and treatability...

 to detect the presence of bile
Bile
Bile or gall is a bitter-tasting, dark green to yellowish brown fluid, produced by the liver of most vertebrates, that aids the process of digestion of lipids in the small intestine. In many species, bile is stored in the gallbladder and upon eating is discharged into the duodenum...

 in urine
Urine
Urine is a typically sterile liquid by-product of the body that is secreted by the kidneys through a process called urination and excreted through the urethra. Cellular metabolism generates numerous by-products, many rich in nitrogen, that require elimination from the bloodstream...

. To carry out the test, the urine is passed through the same filter paper several times. The filter paper is then dried, and a drop of nitric acid
Nitric acid
Nitric acid , also known as aqua fortis and spirit of nitre, is a highly corrosive and toxic strong acid.Colorless when pure, older samples tend to acquire a yellow cast due to the accumulation of oxides of nitrogen. If the solution contains more than 86% nitric acid, it is referred to as fuming...

 is added. In the presence of bile, the characteristic bile pigment colours appear (a yellow spot with rings of red, violet, blue and green around it).

The test is named after Ottomar Rosenbach
Ottomar Rosenbach
Ottomar Ernst Felix Rosenbach was a German physician.Krappitz was a Silesian city where his father, Samuel Rosenbach, practised medicine. He received his education at the universities of Berlin and Breslau . His studies were interrupted by the Franco-Prussian war, in which he took an active part...

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