Plant genetics
Encyclopedia
Plant genetics is a very broad term. There are many facets of genetics
Genetics
Genetics , a discipline of biology, is the science of genes, heredity, and variation in living organisms....

 in general, and of course there are many facets to plants.
The definition of genetics is the branch of biology
Biology
Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, origin, evolution, distribution, and taxonomy. Biology is a vast subject containing many subdivisions, topics, and disciplines...

 that deals with heredity
Heredity
Heredity is the passing of traits to offspring . This is the process by which an offspring cell or organism acquires or becomes predisposed to the characteristics of its parent cell or organism. Through heredity, variations exhibited by individuals can accumulate and cause some species to evolve...

, especially the mechanisms of hereditary transmission and the variation of inherited characteristics among similar or related organisms. And the definition of a plant is any of various photosynthetic, eukaryotic, multicellular organisms of the kingdom Plantae characteristically producing embryos, containing chloroplasts, having cell
Cell (biology)
The cell is the basic structural and functional unit of all known living organisms. It is the smallest unit of life that is classified as a living thing, and is often called the building block of life. The Alberts text discusses how the "cellular building blocks" move to shape developing embryos....

 walls
Cell wall
The cell wall is the tough, usually flexible but sometimes fairly rigid layer that surrounds some types of cells. It is located outside the cell membrane and provides these cells with structural support and protection, and also acts as a filtering mechanism. A major function of the cell wall is to...

 which contain cellulose
Cellulose
Cellulose is an organic compound with the formula , a polysaccharide consisting of a linear chain of several hundred to over ten thousand β linked D-glucose units....

, and lacking the power of locomotion
Animal locomotion
Animal locomotion, which is the act of self-propulsion by an animal, has many manifestations, including running, swimming, jumping and flying. Animals move for a variety of reasons, such as to find food, a mate, or a suitable microhabitat, and to escape predators...

. Although there has been a revolution in the biological sciences in the past twenty years, there is still a great deal that remains to be discovered. The completion of the sequencing of the human genome
Human genome
The human genome is the genome of Homo sapiens, which is stored on 23 chromosome pairs plus the small mitochondrial DNA. 22 of the 23 chromosomes are autosomal chromosome pairs, while the remaining pair is sex-determining...

, as well as the genome
Genome
In modern molecular biology and genetics, the genome is the entirety of an organism's hereditary information. It is encoded either in DNA or, for many types of virus, in RNA. The genome includes both the genes and the non-coding sequences of the DNA/RNA....

s of some agriculturally and scientifically important plants (for example rice
Rice
Rice is the seed of the monocot plants Oryza sativa or Oryza glaberrima . As a cereal grain, it is the most important staple food for a large part of the world's human population, especially in East Asia, Southeast Asia, South Asia, the Middle East, and the West Indies...

), has increased the possibilities of genetic research immeasurably.

Features of plant biology

Plants differ from animals in a few ways that make the study of plant genetics interesting. Like mitochondria, chloroplasts have their own DNA, complicating pedigrees somewhat. Like animals, plants have somatic mutations regularly, but these mutations can contribute to the germ line with ease, since flowers develop at the ends of branches composed of somatic cells. People have known of this for centuries, and mutant branches are called "sports". If the fruit on the sport is economically desirable, a new cultivar
Cultivar
A cultivar'Cultivar has two meanings as explained under Formal definition. When used in reference to a taxon, the word does not apply to an individual plant but to all those plants sharing the unique characteristics that define the cultivar. is a plant or group of plants selected for desirable...

 may be obtained.

Some plant species are capable of self-fertilization, and some are nearly exclusively self-fertilizers. This means that a plant can be both mother and father to its offspring, a rare occurrence in the animals. Scientists and hobbyists attempting to make crosses between different plants must take special measures to prevent the plants from self-fertilizing.

Plants are generally more capable of surviving, and indeed flourishing, as polyploids. Polyploidy, the presence of extra sets of chromosomes, is not usually compatible with life in animals. In plants, polyploid individuals are created frequently by a variety of processes, and once created usually cannot cross back to the parental type. Polyploid individuals, if capable of self-fertilizing, can give rise to a new genetically distinct lineage, which can be the start of a new species. This is often called "instant speciation". Polyploids generally have larger fruit, an economically desirable trait, and many human food crops, including wheat
Wheat
Wheat is a cereal grain, originally from the Levant region of the Near East, but now cultivated worldwide. In 2007 world production of wheat was 607 million tons, making it the third most-produced cereal after maize and rice...

, maize
Maize
Maize known in many English-speaking countries as corn or mielie/mealie, is a grain domesticated by indigenous peoples in Mesoamerica in prehistoric times. The leafy stalk produces ears which contain seeds called kernels. Though technically a grain, maize kernels are used in cooking as a vegetable...

, potatoes, peanuts
Peanuts
Peanuts is a syndicated daily and Sunday American comic strip written and illustrated by Charles M. Schulz, which ran from October 2, 1950, to February 13, 2000, continuing in reruns afterward...

, strawberries and tobacco
Tobacco
Tobacco is an agricultural product processed from the leaves of plants in the genus Nicotiana. It can be consumed, used as a pesticide and, in the form of nicotine tartrate, used in some medicines...

, are either accidentally or deliberately created polyploids.

Hybrids between plant species are easy to create by hand-pollination, and may be more successful on average than hybrids between animal species. Often tens of thousands of offspring from a single cross are raised and tested to obtain a single individual with desired characteristics. People create hybrids for economic and aesthetic reasons, especially with orchids.

DNA

Deoxyribonucleic acid (DNA) is a nucleic acid
Nucleic acid
Nucleic acids are biological molecules essential for life, and include DNA and RNA . Together with proteins, nucleic acids make up the most important macromolecules; each is found in abundance in all living things, where they function in encoding, transmitting and expressing genetic information...

 that contains the genetic instructions used in the development and functioning of all known living organisms and some viruses. The main role of DNA molecules is the long-term storage of information. DNA is often compared to a set of blueprints or a recipe, or a code, since it contains the instructions needed to construct other components of cells, such as proteins and RNA molecules. The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information. Geneticist
Geneticist
A geneticist is a biologist who studies genetics, the science of genes, heredity, and variation of organisms. A geneticist can be employed as a researcher or lecturer. Some geneticists perform experiments and analyze data to interpret the inheritance of skills. A geneticist is also a Consultant or...

s, including plant geneticist
Plant geneticist
A plant geneticist is a scientist involved with the study of genetics in botany. Typical work is done with genes in order to isolate and then develop certain plant traits...

s, use this sequencing of DNA to their advantage as they splice and delete certain genes and regions of the DNA molecule to produce a different or desired genotype
Genotype
The genotype is the genetic makeup of a cell, an organism, or an individual usually with reference to a specific character under consideration...

 and thus, also producing a different phenotype
Phenotype
A phenotype is an organism's observable characteristics or traits: such as its morphology, development, biochemical or physiological properties, behavior, and products of behavior...

.

Gregor Mendel

Gregor Mendel
Gregor Mendel
Gregor Johann Mendel was an Austrian scientist and Augustinian friar who gained posthumous fame as the founder of the new science of genetics. Mendel demonstrated that the inheritance of certain traits in pea plants follows particular patterns, now referred to as the laws of Mendelian inheritance...

 was an Augustinian
Augustinians
The term Augustinians, named after Saint Augustine of Hippo , applies to two separate and unrelated types of Catholic religious orders:...

 priest
Priest
A priest is a person authorized to perform the sacred rites of a religion, especially as a mediatory agent between humans and deities. They also have the authority or power to administer religious rites; in particular, rites of sacrifice to, and propitiation of, a deity or deities...

 and scientist, and is often called the father of genetics for his study of the inheritance of certain traits in pea plants. Mendel showed that the inheritance of these traits follows particular laws
Mendelian inheritance
Mendelian inheritance is a scientific description of how hereditary characteristics are passed from parent organisms to their offspring; it underlies much of genetics...

, which were later named after him. The significance of Mendel's work was not recognized until the turn of the 20th century. Its rediscovery prompted the foundation of the discipline of genetics allows geneticists today to accurately predict the outcome of such crosses and in determining the phenotypical effects of the crosses. He was born on 20 July 1822 and died on 6 January 1884 from chronic nephritis. His nationality was Austria-Hungary. His field was Genetics and went to the Abbey of St. Thomas in Brno. He was well known for discovering genetics.

Modern ways to genetically modify plants

There are two predominant procedures of transforming
Transformation (genetics)
In molecular biology transformation is the genetic alteration of a cell resulting from the direct uptake, incorporation and expression of exogenous genetic material from its surroundings and taken up through the cell membrane. Transformation occurs naturally in some species of bacteria, but it can...

 genes in organisms: the "Gene gun
Gene gun
A gene gun or a biolistic particle delivery system, originally designed for plant transformation, is a device for injecting cells with genetic information. The payload is an elemental particle of a heavy metal coated with plasmid DNA...

" method and the Agrobacterium
Agrobacterium
Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus...

method.

"Gene gun" method

The "Gene Gun" method is also referred to as "biolistics" (ballistics
Ballistics
Ballistics is the science of mechanics that deals with the flight, behavior, and effects of projectiles, especially bullets, gravity bombs, rockets, or the like; the science or art of designing and accelerating projectiles so as to achieve a desired performance.A ballistic body is a body which is...

 using biological components). This technique is used for in vivo
Vivo
-Computer and technology:* Video In Video Out* Vivo Software, streaming format, acquired in 1998 by RealNetworks* VivoActive, Vivo Software's video format* Vivo S.A., a major Brazilian mobile phone company...

, within a living organism, transformation and has been especially useful in transforming monocot
Monocotyledon
Monocotyledons, also known as monocots, are one of two major groups of flowering plants that are traditionally recognized, the other being dicotyledons, or dicots. Monocot seedlings typically have one cotyledon , in contrast to the two cotyledons typical of dicots...

 species like corn
Maize
Maize known in many English-speaking countries as corn or mielie/mealie, is a grain domesticated by indigenous peoples in Mesoamerica in prehistoric times. The leafy stalk produces ears which contain seeds called kernels. Though technically a grain, maize kernels are used in cooking as a vegetable...

, otherwise known as maize, and rice.
This approach literally shoots genes into plant cells and plant cell chloroplasts. DNA is coated onto small particles of gold or tungsten
Tungsten
Tungsten , also known as wolfram , is a chemical element with the chemical symbol W and atomic number 74.A hard, rare metal under standard conditions when uncombined, tungsten is found naturally on Earth only in chemical compounds. It was identified as a new element in 1781, and first isolated as...

 approximately two micrometres in diameter. The particles are placed in a vacuum chamber and the plant tissue to be engineered is placed below the chamber. The particles are propelled at high velocity using a short pulse of high pressure helium gas, and hit a fine mesh baffle placed above the tissue while the DNA coating continues into any target cell or tissue
Tissue (biology)
Tissue is a cellular organizational level intermediate between cells and a complete organism. A tissue is an ensemble of cells, not necessarily identical, but from the same origin, that together carry out a specific function. These are called tissues because of their identical functioning...

.

Agrobacterium method

Transformation via Agrobacterium has been successfully practiced in dicots
Dicotyledon
The dicotyledons, also known as dicots, are a group of flowering plants whose seed typically has two embryonic leaves or cotyledons. There are around 199,350 species within this group...

, i.e. broadleaf plants, such as soybeans and tomatoes, for many years. Recently it has been adapted and is now effective in monocots like grasses, including corn and rice. In general, the Agrobacterium method is considered preferable to the gene gun, because of a greater frequency
Frequency
Frequency is the number of occurrences of a repeating event per unit time. It is also referred to as temporal frequency.The period is the duration of one cycle in a repeating event, so the period is the reciprocal of the frequency...

 of single-site insertions of the foreign DNA, which allows for easier monitoring. In this method, the tumor
Tumor
A tumor or tumour is commonly used as a synonym for a neoplasm that appears enlarged in size. Tumor is not synonymous with cancer...

 inducing (Ti) region is removed from the T-DNA (transfer DNA) and replaced with the desired gene and a marker, which is then inserted into the organism. This may involve direct inoculation of the tissue with a culture of transformed Agrobacterium, or inoculation following treatment with micro-projectile bombardment, which wounds the tissue. Wounding of the target tissue causes the release of phenolic compounds by the plant, which induces invasion of the tissue by Agrobacterium. Because of this, microprojectile bombardment often increases the efficiency of infection with Agrobacterium. The marker is used to find the organism which has successfully taken up the desired gene. Tissues of the organism are then transferred to a medium containing an antibiotic
Antibiotic
An antibacterial is a compound or substance that kills or slows down the growth of bacteria.The term is often used synonymously with the term antibiotic; today, however, with increased knowledge of the causative agents of various infectious diseases, antibiotic has come to denote a broader range of...

 or herbicide
Herbicide
Herbicides, also commonly known as weedkillers, are pesticides used to kill unwanted plants. Selective herbicides kill specific targets while leaving the desired crop relatively unharmed. Some of these act by interfering with the growth of the weed and are often synthetic "imitations" of plant...

, depending on which marker was used. The Agrobacterium
Agrobacterium
Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus...

present is also killed by the antibiotic. Only tissues expressing the marker will survive and possess the gene of interest. Thus, subsequent steps in the process will only use these surviving plants. In order to obtain whole plants from these tissues, they are grown under controlled environmental conditions in tissue culture
Plant tissue culture
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation...

. This is a process of a series of media, each containing nutrients and hormones. Once the plants are grown and produce seed, the process of evaluating the progeny
Progeny
Progeny can refer to:*A genetic descendant or offspring*An academic progeny Other uses*Progeny Linux Systems*Progeny - an episode of the television series Stargate Atlantis...

 begins. This process entails selection of the seeds with the desired traits and then retesting and growing to make sure that the entire process has been completed successfully with the desired results.

Benefits of genetically engineered crops

The use of genetically engineered crops has helped many farmers deal with pest problems that reduce their crop production. The impact of pest-resistant crops has led to a much higher yield for farmers in today's world. They can use less pesticides which reduces the chemicals that they put into the ground. Certain engineered crops such as Roundup Ready Corn, a patented transgenic maize
Transgenic maize
Genetically modified maize has been deliberately genetically modified to have agronomically desirable traits. Traits that have been engineered into corn include resistance to herbicides and resistance to insect pests, the latter being achieved by incorporation of a gene that codes for the...

 variety, has led to farmers all over the world and in the United States to increase crop yield exponentially in recent years. Farmers can use a Roundup herbicide to kill weeds, yet the genetically engineered corn is resistant to the herbicide and is left unaffected. Thus, fields are produced that are virtually weed free.
Genetically engineered crops can also benefit farmers when dealing with potentially harmful viruses and bacteria. In the case of the 1990s a mutant strain of virus was decimating the commercial corn fields of the United States. Scientists found a virus resistant strain of maize in the highlands of Mexico and extracted the part of the maize's genome that coded for resistance against the virus and incorporated it into their existing strain of commercial corn. This allowed the commercial strain to produce progeny that were resistant to the virus. Thus, the crops were saved from decimation.

Potential detrimental effects of genetically engineered plants

According to Vaughan A. Hilder and Donald Boulter at the Department of Biological Sciences, University of Durham, there have been serious failures in resistance to targeted pests in Bt cotton; most plant-derived resistance factors produce chronic rather than acute effects; and many serious pests are simply not susceptible to known resistance factors. According to John E. Berringer the outcome of releasing genetically modified organisms into the environment is still not known.

External links

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