Niccolò Gattilusio was the first member of the Gattilusio family to rule the city of Aenos

Aenus (Thrace)

Aenus , modern Enez in Turkey, was an ancient Greek city on the southeastern coast of Thrace. Formerly called Poltyobria , it was located near the mouth of the Hebrus River, not far from the Melas Gulf , which is formed by the Thracian Chersonesus to the east...

 (modern Enez

Enez

Enez is a district of Edirne Province, Turkey, as well as the name of the center-town of the district. Its ancient name was Aenus. The mayor is Ahmet Çayır . The population is 3,826 as of 2010....

 in Turkey

Turkey

Turkey , known officially as the Republic of Turkey , is a Eurasian country located in Western Asia and in East Thrace in Southeastern Europe...

).

The Gattilusio family came from the Republic of Genoa

Republic of Genoa

The Most Serene Republic of Genoa |Ligurian]]: Repúbrica de Zêna) was an independent state from 1005 to 1797 in Liguria on the northwestern Italian coast, as well as Corsica from 1347 to 1768, and numerous other territories throughout the Mediterranean....

. The parents of Niccolò and his brother Francesco I of Lesbos

Francesco I of Lesbos

Francesco I Gattilusio, Lord of Lesbos was the first member of the Gattilusio family to rule the Aegean Island of Lesbos.-Freebooter:...

 are not known.

He accompanied his brother in his conquests and was awarded with the mentioned Island.

He died unmarried and without issue and was succeeded by his grand-nephew Palamede of Ainos.