Mycobacterium conceptionense
Encyclopedia
Mycobacterium conceptionense is a non pigmented rapidly growing mycobacterium
was first isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. Etymology
: conceptionense, pertaining to Hôpital de la Conception, the hospital
where the first strain
was isolated.
Colony characteristics
Physiology
days at temperatures between 25 and 37°C, optimally at 30°C. No growth occurs at 42°C.
Differential characteristics
Mycobacterium
Mycobacterium is a genus of Actinobacteria, given its own family, the Mycobacteriaceae. The genus includes pathogens known to cause serious diseases in mammals, including tuberculosis and leprosy...
was first isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. Etymology
Etymology
Etymology is the study of the history of words, their origins, and how their form and meaning have changed over time.For languages with a long written history, etymologists make use of texts in these languages and texts about the languages to gather knowledge about how words were used during...
: conceptionense, pertaining to Hôpital de la Conception, the hospital
Hospital
A hospital is a health care institution providing patient treatment by specialized staff and equipment. Hospitals often, but not always, provide for inpatient care or longer-term patient stays....
where the first strain
Strain (biology)
In biology, a strain is a low-level taxonomic rank used in three related ways.-Microbiology and virology:A strain is a genetic variant or subtype of a micro-organism . For example, a "flu strain" is a certain biological form of the influenza or "flu" virus...
was isolated.
Description
Microscopy- Acid-fastAcid-fastAcid-fastness is a physical property of certain bacteria, specifically their resistance to decolorization by acids during staining procedures.Acid-fast organisms are difficult to characterize using standard microbiological techniques Acid-fastness is a physical property of certain bacteria,...
and gram-positiveGram-positiveGram-positive bacteria are those that are stained dark blue or violet by Gram staining. This is in contrast to Gram-negative bacteria, which cannot retain the crystal violet stain, instead taking up the counterstain and appearing red or pink...
bacilli.
Colony characteristics
- Colonies are non pigmented.
Physiology
- Colonies appear on 5% sheep blood agarAgarAgar or agar-agar is a gelatinous substance derived from a polysaccharide that accumulates in the cell walls of agarophyte red algae. Throughout history into modern times, agar has been chiefly used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture medium...
, Middlebrook 7H10 agar, and egg-based Lowenstein-Jensen slants in 2 to 5
days at temperatures between 25 and 37°C, optimally at 30°C. No growth occurs at 42°C.
- The type strain is susceptible in vitro to imipenem, minocycline, doxycycline, clarithromycin, erythromycin, azithromycin, amikacin, ciprofloxacin, ofloxacin, sparfloxacin, and amoxicillinclavulanate and resistant to penicillin, amoxicillin, and vancomycin.
Differential characteristics
- M. conceptionense shares 99.7% 16S rRNA (4-bp difference) and 97.0% rpoB gene sequence similarity with Mycobacterium porcinumMycobacterium porcinumMycobacterium porcinum is a species of Mycobacterium....
, the nearest species.