Murashige and Skoog medium
Overview
 
Murashige and Skoog medium or (MSO or MS0 (MS-zero)) is a plant
Plant
Plants are living organisms belonging to the kingdom Plantae. Precise definitions of the kingdom vary, but as the term is used here, plants include familiar organisms such as trees, flowers, herbs, bushes, grasses, vines, ferns, mosses, and green algae. The group is also called green plants or...

 growth medium
Growth medium
A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens.There are different types of media for growing different types of cells....

 used in the laboratories for cultivation of plant cell culture
Cell culture
Cell culture is the complex process by which cells are grown under controlled conditions. In practice, the term "cell culture" has come to refer to the culturing of cells derived from singlecellular eukaryotes, especially animal cells. However, there are also cultures of plants, fungi and microbes,...

. MSO was invented by plant scientists Toshio Murashige
Toshio Murashige
Toshio Murashige is a professor emeritus of University of California Riverside in plant biology.He is most widely known for his efforts in creating the plant tissue culture medium known as Murashige and Skoog medium.- External links :*...

 and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. Along with its modifications, it is the most commonly used medium in plant tissue culture
Plant tissue culture
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation...

 experiments in laboratorium.

As Skoog's doctoral student, Murashige originally set out to find an as-yet undiscovered growth hormone present in tobacco juice.
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