A gate in cytometry

Cytometry

Cytometry is a general name for a group of biological methods used to measure various parameters of cells. Parameters which can be measured by cytometric methods are cell size, the stage of the cell cycle, the DNA content of the cell, the existence or absence of specific proteins on the cell...

is a set of value limits (boundaries) that serve to isolate a specific group of cytometric

Cytometry

Cytometry is a general name for a group of biological methods used to measure various parameters of cells. Parameters which can be measured by cytometric methods are cell size, the stage of the cell cycle, the DNA content of the cell, the existence or absence of specific proteins on the cell...

 events from a large set. Gates can be defined by discrimination analysis, or can simply be drawn around a given set of data points on a printout and then converted to a computer-useful form. Gates can be implemented with a physical blinder. Gates may be used either to selectively gather data or to segregate data for analysis.

Division

Gates are divided mathematically into inclusive gates and exclusive gates. Inclusive gates select data that falls within the limits set, while exclusive gates select data that falls outside the limits.

Live gate

A live gate is a term used for a process that prevents the acquisition by the computer of non-selected data from the flow cytometer.

External links

• "General Flow Cytometry Glossary and Cell Cycle Analysis Terminology" The Janis V. Giorgi Flow Cytometry Laboratory, UCLA
• Osborne, G. W. (2000) "Regions and Gates" Flow Cytometry Software Workshop: 2000, page 3