Brian S. Hooker, PhD, PE, is a bioengineer and the team leader for the High Throughput Biology Team and Operations Manager of the DOE Genomics: Genomes to Life (GTL) Center for Molecular and Cellular Systems at the Pacific Northwest National Laboratory

Pacific Northwest National Laboratory

Pacific Northwest National Laboratory is one of the United States Department of Energy National Laboratories, managed by the Department of Energy's Office of Science. The main campus of the laboratory is in Richland, Washington....

 (PNNL). Hooker also is credited as a co-inventor for five patents.

Education

In 1985, Dr. Hooker earned his bachelor of science degree in chemical engineering

Chemical engineering

Chemical engineering is the branch of engineering that deals with physical science , and life sciences with mathematics and economics, to the process of converting raw materials or chemicals into more useful or valuable forms...

, from California State Polytechnic University, Pomona, California

Pomona, California

-2010:The 2010 United States Census reported that Pomona had a population of 149,058, a slight decline from the 2000 census population. The population density was 6,491.2 people per square mile...

. He earned his master's of science degree in 1988 and his doctorate in 1990, both in biochemical engineering

Biochemical engineering

Biochemical engineering is a branch of chemical engineering or biological engineering that mainly deals with the design and construction of unit processes that involve biological organisms or molecules, such as bioreactors...

, from Washington State University

Washington State University

Washington State University is a public research university based in Pullman, Washington, in the Palouse region of the Pacific Northwest. Founded in 1890, WSU is the state's original and largest land-grant university...

, in Pullman, Washington

Pullman, Washington

Pullman is the largest city in Whitman County, Washington, United States. The population was 24,675 at the 2000 census and 29,799 according to the 2010 census...

.

Research

Hooker manages applied plant and fungal molecular biology research projects, including development of plant-based biosensors and transgenic production systems for human pharmaceutical proteins and industrial enzyme

Enzyme

Enzymes are proteins that catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates...

s at the Pacific Northwest National Laboratory, where systems biology

Systems biology

Systems biology is a term used to describe a number of trends in bioscience research, and a movement which draws on those trends. Proponents describe systems biology as a biology-based inter-disciplinary study field that focuses on complex interactions in biological systems, claiming that it uses...

 researchers are focused on understanding gene and protein networks involved in individual cell signaling, communication between cells in communities, and cellular metabolic pathways. Hooker has also been involved in research on microbial kinetics and transport mathematical modeling, design, development, and support for biological destruction of chlorinated organic hydrocarbons, development of tP4 transgenic plant protein production platform technology, and development of the RT3D bioremediation/natural attenuation software package.

Recognition

Hooker earned the Battelle Entrepreneurial Award in 2001, and a Federal Laboratory Consortium Recognition Award in 1999, for his work on “Reactive Transport in 3-Dimensions.”

Selected publications

• Dai Z, BS Hooker, RD Quesenberry, and J Gao. “Expression of Trichoderma reesei exo-cellobiohydrolase I (CBH I) in transgenic tobacco leaves and calli”, Applied Biochemistry and Biotechnology, 1999, 77-79(9):689-699
• Dai Z, BS Hooker, DB Anderson, and SR Thomas.“Improved plant-based production of E1 endoglucanase using potato:expression optimization and tissue targeting”, Molecular Breeding, 2000, 6(3):277-285.
• Gao J, BS Hooker, and DB Anderson. 2004. “Expression of Functional Human Coagulation Factor XIII A-domain in Plant Cell Suspensions and Whole Plants.” Protein Expression and Purification 37(1):89-96.
• Hooker, Brian S., Hettich, Robert L., Hurst, Gregory B., Kennel, Stephen J., Lankford, Patricia K., Chiann-Tso Lin, Lye Meng Markillie, Mayer-Clumbridge, M. Uljana, Pelletier, Dale A., Liang, Shi, Squier, Thomas C., Strader, Michael B., VerBerkmoes, Nathan C., "Isolation and Characterization of Protein Complexes from Shewanella oneidensis and Rhodopseudomonas palustris", Genomics: GTL Contractor—Grantee Workshop II, Washington, DC, February 29-March 2, 2004
• Trelka, Jeffrey A., Hooker, Brian S. "Specific Carbohydrate Dietary Trial: Understanding the Effectiveness of a Specific Carbohydrate Dietary Intervention In Autistic Children", 2004
• Markillie LM, CT Lin, JN Adkins, DL Auberry, EA Hill, BS Hooker, PA Moore, RJ Moore, L Shi, HS Wiley, and V Kery, “A simple protein complex purification and identification method for high throughput mapping of protein interaction networks”, Journal of Proteome Research, 2005
• Liang Shi, Jiann-Trzwo Lin, Lye M. Markillie, Thomas C. Squier, and Brian S. Hooker, “Overexpression of multi-heme C-type cytochromes” - Expression of recombinant decaheme cytochrome MtrA in Shewanella oneidensis MR-1 by a directional TOPO cloning-based system. BioTechniques, 2005, 38(2):297-9.

External links

• SysBio.org - 'Brian S. Hooker, Ph.D.' (profile) Pacific Northwest National Laboratory
  Pacific Northwest National Laboratory
  Pacific Northwest National Laboratory is one of the United States Department of Energy National Laboratories, managed by the Department of Energy's Office of Science. The main campus of the laboratory is in Richland, Washington....