- This page is about the bacterial genus. For the class, see Bacilli
Bacilli refers to a taxonomic class of bacteria. It includes two orders, Bacillales and Lactobacillales, which contain several well-known pathogens like Bacillus anthracis .-Ambiguity:...
.
Bacillus is a
genusIn biology, a genus is a low-level taxonomic rank used in the biological classification of living and fossil organisms, which is an example of definition by genus and differentia...
of
Gram-positiveGram-positive bacteria are those that are stained dark blue or violet by Gram staining. This is in contrast to Gram-negative bacteria, which cannot retain the crystal violet stain, instead taking up the counterstain and appearing red or pink...
, rod-shaped
bacteriaBacteria are a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria have a wide range of shapes, ranging from spheres to rods and spirals...
and a member of the division
FirmicutesThe Firmicutes are a phylum of bacteria, most of which have Gram-positive cell wall structure. A few, however, such as Megasphaera, Pectinatus, Selenomonas and Zymophilus, have a porous pseudo-outer-membrane that causes them to stain Gram-negative...
.
Bacillus species can be obligate aerobes or facultative anaerobes, and test positive for the
enzymeEnzymes are proteins that catalyze chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates...
catalaseCatalase is a common enzyme found in nearly all living organisms that are exposed to oxygen, where it catalyzes the decomposition of hydrogen peroxide to water and oxygen...
. Ubiquitous in nature,
Bacillus includes both free-living and
pathogenA pathogen gignomai "I give birth to") or infectious agent — colloquially, a germ — is a microbe or microorganism such as a virus, bacterium, prion, or fungus that causes disease in its animal or plant host...
ic species. Under stressful environmental conditions, the cells produce oval
endosporeAn endospore is a dormant, tough, and temporarily non-reproductive structure produced by certain bacteria from the Firmicute phylum. The name "endospore" is suggestive of a spore or seed-like form , but it is not a true spore . It is a stripped-down, dormant form to which the bacterium can reduce...
s that can stay dormant for extended periods. These characteristics originally defined the genus, but not all such species are closely related, and many have been moved to other genera.
Industrial significance
Many
Bacillus species are able to
secreteSecretion is the process of elaborating, releasing, and oozing chemicals, or a secreted chemical substance from a cell or gland. In contrast to excretion, the substance may have a certain function, rather than being a waste product...
large quantities of enzymes.
Bacillus amyloliquefaciensBacillus amyloliquefaciens is a species of bacteria that is the source of the BamH1 restriction enzyme. It also synthesizes a natural antibiotic protein barnase, a widely studied ribonuclease that forms a famously tight complex with its intracellular inhibitor barstar.-Discovery and name:B...
is the source of a natural antibiotic protein
barnaseBarnase is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. It is synthesized and secreted by the bacterium Bacillus amyloliquefaciens, but is lethal to the cell when expressed without its inhibitor barstar...
(a
ribonucleaseRibonuclease is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7 and 3.1 classes of enzymes.-Function:All organisms studied contain...
), alpha amylase used in starch hydrolysis, the
proteaseA protease is any enzyme that conducts proteolysis, that is, begins protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain forming the protein....
subtilisinSubtilisin is a non-specific protease initially obtained from Bacillus subtilis.Subtilisins belong to subtilases, a group of serine proteases that initiate the nucleophilic attack on the peptide bond through a serine residue at the active site. They are physically and chemically...
used with detergents, and the BamH1
restriction enzymeA Restriction Enzyme is an enzyme that cuts double-stranded DNA at specific recognition nucleotide sequences known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses...
used in DNA research.
A portion of the
Bacillus thuringiensisBacillus thuringiensis is a Gram-positive, soil-dwelling bacterium, commonly used as a biological pesticide; alternatively, the Cry toxin may be extracted and used as a pesticide. B...
genome was incorporated into corn (and cotton) crops. The resulting
GMOA GMO is a genetically modified organism.GMO may also refer to:* Gell-Mann–Okubo mass formula in particle physics* General Medical Officer, a designation for United States Army soldiers* Generalised molecular orbital theory, in chemistry...
s are therefore resistant to some insect pests.
Use as model organism
Bacillus subtilisBacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium commonly found in soil. A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate...
is one of the best understood prokaryotes, in terms of molecular biology and cell biology. Its superb genetic amenability and relatively large size have provided the powerful tools required to investigate a bacterium from all possible aspects. Recent improvements in fluorescence microscopy techniques have provided novel and amazing insight into the dynamic structure of a single cell organism. Research on
Bacillus subtilisBacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium commonly found in soil. A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate...
has been at the forefront of bacterial molecular biology and cytology, and the organism is a model for differentiation, gene/protein regulation, and cell cycle events in bacteria.
Clinical significance
Two
Bacillus species are considered medically significant:
B. anthracisBacillus anthracis is the pathogen of the Anthrax acute disease. It is a Gram-positive, spore-forming, rod-shaped bacterium, with a width of 1-1.2µm and a length of 3-5µm. It can be grown in an ordinary nutrient medium under aerobic or anaerobic conditions.It is one of few bacteria known to...
, which causes
anthraxAnthrax is an acute disease caused by the bacterium Bacillus anthracis. Most forms of the disease are lethal, and it affects both humans and other animals...
, and
B. cereusBacillus cereus is an endemic, soil-dwelling, Gram-positive, rod-shaped, beta hemolytic bacterium. Some strains are harmful to humans and cause foodborne illness, while other strains can be beneficial as probiotics for animals...
, which causes a
foodborne illnessFoodborne illness is any illness resulting from the consumption of contaminated food, pathogenic bacteria, viruses, or parasites that contaminate food, as well as chemical or natural toxins such as poisonous mushrooms.-Causes:Foodborne illness usually arises from improper handling, preparation, or...
similar to that of
StaphylococcusStaphylococcus is a genus of Gram-positive bacteria. Under the microscope they appear round , and form in grape-like clusters....
. A third species,
B. thuringiensis, is an important
insectInsects are a class of living creatures within the arthropods that have a chitinous exoskeleton, a three-part body , three pairs of jointed legs, compound eyes, and two antennae...
pathogen, and is sometimes used to control insect pests. The
type speciesIn biological nomenclature, a type species is both a concept and a practical system which is used in the classification and nomenclature of animals and plants. The value of a "type species" lies in the fact that it makes clear what is meant by a particular genus name. A type species is the species...
is
B. subtilisBacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium commonly found in soil. A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate...
, an important
model organismA model organism is a non-human species that is extensively studied to understand particular biological phenomena, with the expectation that discoveries made in the organism model will provide insight into the workings of other organisms. Model organisms are in vivo models and are widely used to...
. It is also a notable food spoiler, causing ropiness in bread and related food. Some environmental and commercial strains
B. coagulansBacillus coagulans is a lactic acid-forming bacterial species within the genus Bacillus. The organism was first isolated and described in 1932 and was elaborated in the fifth edition of Bergey's Manual of Determinative Bacteriology. It was initially considered to be a spore-forming Lactobacillus...
may play a role in food spoilage of highly acidic, tomato based products.
An easy way to isolate
Bacillus is by placing nonsterile soil in a
test tubeA test tube, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top, usually with a rounded U-shaped bottom....
with water, shaking, placing in melted
mannitol salt agarMannitol salt agar or MSA is a commonly used growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. This medium is important in medical laboratories by distinguishing pathogenic microbes in a short period of time...
, and incubating at room temperature for at least a day. Colonies are usually large, spreading and irregularly-shaped. Under the microscope, the
Bacillus cells appear as rods, and a substantial portion usually contain an oval
endosporeAn endospore is a dormant, tough, and temporarily non-reproductive structure produced by certain bacteria from the Firmicute phylum. The name "endospore" is suggestive of a spore or seed-like form , but it is not a true spore . It is a stripped-down, dormant form to which the bacterium can reduce...
at one end, making it bulge.
The cell wall
The cell wall of
Bacillus is a structure on the outside of the cell that forms the second barrier between the bacterium and the environment, and at the same time maintains the rod shape and withstands the pressure generated by the cell's turgor. The cell wall is composed of
teichoicTeichoic acids are bacterial polysaccharides of glycerol phosphate or ribitol phosphate linked via phosphodiester bonds.-Location and structure:...
and teichuronic acids.
B. subtilis is the first bacterium for which the role of an
actinActin is a globular, roughly 42-kDa moonlighting protein found in all eukaryotic cells where it may be present at concentrations of over 100 μM. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans...
-like
cytoskeletonThe cytoskeleton is a cellular "scaffolding" or "skeleton" contained within a cell's cytoplasm and is made out of protein. The cytoskeleton is present in all cells; it was once thought to be unique to eukaryotes, but recent research has identified the prokaryotic cytoskeleton...
in cell shape determination and
peptidoglycanPeptidoglycan, also known as murein, is a polymer consisting of sugars and amino acids that forms a mesh-like layer outside the plasma membrane of bacteria , forming the cell wall. The sugar component consists of alternating residues of β- linked N-acetylglucosamine and N-acetylmuramic acid...
synthesis was identified, and for which the entire set of peptidoglycan-synthesizing enzymes was localised. The role of the cytoskeleton in shape generation and maintenance is important.
Phylogeny
The genus
Bacillus was coined in 1835 by
Christian Gottfried EhrenbergChristian Gottfried Ehrenberg , German naturalist, zoologist, comparative anatomist, geologist, and microscopist, was one of the most famous and productive scientists of his time.- Early collections :...
(who coined the genus
BacteriumThe genus Bacterium was a taxon described in 1828 by Christian Gottfried Ehrenberg. The type species was later changed from Bacterium triloculare to Bacterium coli as it was lost...
seven years prior) to contain rod-shaped bacteria, later amended by
Ferdinand CohnFerdinand Julius Cohn was a German biologist.Cohn was born in Breslau in the Prussian Province of Silesia. At the age of 10 he suffered hearing impairment. He received a degree in botany in 1847 at the age of nineteen at the University of Berlin. He was a teacher and researcher at University of...
to spore-forming, Gram-positive/variable, rod-shaped bacteria. Like other genera associated with the early history of microbiology, such as
PseudomonasPseudomonas is a genus of gammaproteobacteria, belonging to the family Pseudomonadaceae containing 191 validly described species.Recently, 16S rRNA sequence analysis has redefined the taxonomy of many bacterial species. As a result, the genus Pseudomonas includes strains formerly classified in the...
or
VibrioVibrio is a genus of Gram-negative bacteria possessing a curved rod shape, several species of which can cause foodborne infection, usually associated with eating undercooked seafood. Typically found in saltwater, Vibrio are facultative anaerobes that test positive for oxidase and do not form...
, members of the
Bacillus genus (266 species) are found ubiquitously, and it is one of the genera with the largest
16S16S ribosomal RNA is a component of the 30S subunit of prokaryotic ribosomes. It is approximately 1.5kb in length...
diversity and environmental diversity.
Several studies have tried to reconstruct the phylogeny of the genus. The
Bacillus-specific study with the most diversity covered is by Xu and Cote' using 16S and the ITS region, where they divide the genus into 10 groups, which includes the nested genera
Paenibacillus, Brevibacillus, Geobacillus, Marinibacillus and
Virgibacillus. However, the tree
http://www.arb-silva.de/fileadmin/silva_databases/living_tree/LTP_release_104/LTPs104_SSU_tree.pdf constructed by the living tree project, a collaboration between
ARB-SilvaARB may stand for:In Medicine:* Angiotensin receptor blocker , a medication for treating high blood pressureIn the Military:...
and
LPSNList of Prokaryotic names with Standing in Nomenclature is an online database that maintains and provides accurate name and related information of prokaryotes according to the International Code of Nomenclature of Bacteria , curated by prof...
where a 16S (and 23S if available) tree of all validated species was constructed, the genus
Bacillus contains a very large number of nested taxa and majorly in both 16S and 23S it is paraphyletic to Lactobacillales (
Lactobacillus, Streptococcus, Staphylococcus, Listeria, etc.), due to
Bacillus coahuilensisBacillus coahuilensis is a rod-shaped, Gram-positive, motile, spore-forming bacterium. This species was isolated from water samples taken from a highly saline desert lagoon in Coahuila, Mexico....
and others. A gene concatenation study found similar results to Xu and Cote', but with a much more limited number of species in terms of groups, but used
Listeria as an outgroup, so in light of the ARB tree, it may be "inside-out".
One clade, formed by
B. anthracis,
B. cereus,
B. mycoides,
B. pseudomycoides,
B. thuringiensis and
B. weihenstephanensis under current classification standards, should be a single species (within 97% 16S identity), but due to medical reasons, they are considered separate species, an issue also present for four species of
ShigellaShigella is a genus of Gram-negative, nonspore forming, non-motile, rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella causes disease in primates, but not in other mammals. It is only naturally found in humans and apes. During...
and
Escherichia coliEscherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms . Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls...
.
See also
- Paenibacillus
'Paenibacillus' is a genus of Gram-positive, facultative anaerobic, endospore-forming bacteria, originally included within the genus Bacillus and then reclassified as a separate genus in 1993. Bacteria belonging to this genus have been detected in a variety of environments such as: soil, water,...
, a genus of bacteria that was formerly included in Bacillus
External links
- Bacillus genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID