I am doing a "virtual" project about LTP. Which means I have to write a whole proposal of something I have never than and will probably never do.

I am undecided whether to use in vivo or slice preparations for LTP. Slice preparations will be a LOT easier since I will also need to electroporate (or transfect by virus) siRNA and other drugs.

so the question is:

How long can I keep a slice going?

Ideally I would like to have a preparation that stays good for 2 days after LTP induction. is this possible?

If you have any good references for a protocol that will be welcomed too!

Thanks!