Hi,

I have a data for enzyme malate dehydrogenase at absorbance 340nm and at different times with substrate conc. I have to determine Km and Vmax. I know I have to make lineweaver plot but the only thing I am not getting is which data to use for lineweaver plot.

I have this data

time 0.5 1.0 1.5 2.0 2.5 3.0
abs .033 .056 .079 .102 .122 .138
.036 .063 .089 .116 .138 .154
.040 .069 .099 .128 .150 .168