Hello,

I have to find a way to quantify the distribution of my fluorescence tagged protein in a cell.

I would like to show that upon knockdown/ over-expression of specific proteins which are involved in trafficking, other protein cluster in a specific region whereas in control cells they show a discrete punctuate structure in the cell.

Does anyone know how to do that by using the ImageJ softwarre? I am not sure whether I need a specific plugIn for that.

Can anyone help?

Thanks,
Dayani